A visible dominant marker for insect transgenesis

被引:48
作者
Osanai-Futahashi, Mizuko [1 ]
Ohde, Takahiro [2 ]
Hirata, Junya [2 ]
Uchino, Keiro [1 ]
Futahashi, Ryo [3 ]
Tamura, Toshiki [1 ]
Niimi, Teruyuki [2 ]
Sezutsu, Hideki [1 ]
机构
[1] NIAS, Transgen Silkworm Res Unit, Tsukuba, Ibaraki 3058634, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[3] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan
来源
NATURE COMMUNICATIONS | 2012年 / 3卷
关键词
ARYLALKYLAMINE-N-ACETYLTRANSFERASE; GERMLINE TRANSFORMATION; GENE-EXPRESSION; DROSOPHILA-MELANOGASTER; SILKWORM; SYSTEM; CLONING; YELLOW; BEETLE; EBONY;
D O I
10.1038/ncomms2312
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Transgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori beta-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa.
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页数:9
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