Following the Biocatalytic Activities of Glucose Oxidase by Electrochemically Cross-Linked Enzyme-Pt Nanoparticles Composite Electrodes

被引：70

作者：

Bahshi, Lily ^{[1
]}

Frasconi, Marco ^{[2
]}

Tel-Vered, Ran ^{[1
]}

Yehezkeli, Omer ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

[2] Univ Roma La Sapienza, Dipartimento Chim & Tecnol Sostanze Biologicament, Rome, Italy

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 21期

关键词：

D O I：

10.1021/ac801398m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O-2 oxidation of glucose to gluconic acid and H2O2. The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H2O2. The effect of the composition of the Pt NPs/GOx crosslinked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.

引用

页码：8253 / 8259

页数：7

共 52 条

[1] The influence of decanethiol/4-aminothiophenol mixed monolayers on the electrodeposition of polyaniline thin films [J].

Abaci, S ;

Shannon, C .

ELECTROCHIMICA ACTA, 2005, 50 (14) :2967-2973

[2] Sensing of acetylcholine by a tricomponent-enzyme layered electrode using faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance transduction methods [J].

Alfonta, L ;

Katz, E ;

Willner, I .

ANALYTICAL CHEMISTRY, 2000, 72 (05) :927-935

[3] Metal-dispersed conducting polymer-coated electrode used for oxidase-based biosensors [J].

Arjsiriwat, S ;

Tanticharoen, M ;

Kirtikara, K ;

Aoki, K ;

Somasundrum, M .

ELECTROCHEMISTRY COMMUNICATIONS, 2000, 2 (06) :441-444

[4] Hydrogels of a conducting conjugated polymer as 3-D enzyme electrode [J].

Åsberg, P ;

Inganäs, O .

BIOSENSORS & BIOELECTRONICS, 2003, 19 (03) :199-207

[5] Dopamine-, L-DOPA-, adrenaline-, and noradrenaline-induced growth of Au nanoparticles: Assays for the detection of neurotransmitters and of tyrosinase activity [J].

Baron, R ;

Zayats, M ;

Willner, I .

ANALYTICAL CHEMISTRY, 2005, 77 (06) :1566-1571

[6] DESIGN AND INVITRO STUDIES OF A NEEDLE-TYPE GLUCOSE SENSOR FOR SUBCUTANEOUS MONITORING [J].

BINDRA, DS ;

ZHANG, YN ;

WILSON, GS ;

STERNBERG, R ;

THEVENOT, DR ;

MOATTI, D ;

REACH, G .

ANALYTICAL CHEMISTRY, 1991, 63 (17) :1692-1696

[7] Glucose oxidase electrodes of polyaniline, poly(o-toluidine) and their copolymer as a biosensor:: a comparative study [J].

Borole, DD ;

Kapadi, UR ;

Mahulikar, PP ;

Hundiwale, DG .

POLYMERS FOR ADVANCED TECHNOLOGIES, 2004, 15 (06) :306-312

[8] COVALENT LINKAGE OF GLUCOSE-OXIDASE ON MODIFIED GLASSY-CARBON ELECTRODES - KINETIC PHENOMENA [J].

BOURDILLON, C ;

BOURGEOIS, JP ;

THOMAS, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (12) :4231-4235

[9] Co-immobilization of lactate oxidase and lactate dehydrogenase on conducting polyaniline films [J].

Chaubey, A ;

Pande, KK ;

Singh, VS ;

Malhotra, BD .

ANALYTICA CHIMICA ACTA, 2000, 407 (1-2) :97-103

[10] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .2. METHODS FOR BONDING ELECTRON-TRANSFER RELAYS TO GLUCOSE-OXIDASE AND D-AMINO-ACID OXIDASE [J].

DEGANI, Y ;

HELLER, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (08) :2615-2620

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