Nuclear organization of mammalian genomes: polar chromosome territories build up functionally distinct higher order compartments

被引:233
作者
Sadoni, N
Langer, S
Fauth, C
Bernardi, G
Cremer, T
Turner, BM
Zink, D
机构
[1] Univ Munich, Inst Anthropol & Humangenet, D-80336 Munich, Germany
[2] Stn Zool A Dohrn, I-80121 Naples, Italy
[3] Univ Munich, Inst Anthropol & Humangenet, D-80333 Munich, Germany
[4] Univ Birmingham, Sch Med, Chromatin & Gene Express Grp, Dept Anat, Birmingham B15 2TT, W Midlands, England
基金
英国惠康基金;
关键词
nuclear architecture; genome organization; chromosome structure; nuclear compartments; polar chromosome territories;
D O I
10.1083/jcb.146.6.1211
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We investigated the nuclear higher order compartmentalization of chromatin according to its replication timing (Ferreira et al., 1997) and the relations of this compartmentalization to chromosome structure and the spatial organization of transcription. Our aim was to provide a comprehensive and integrated view on the relations between chromosome structure and functional nuclear architecture. Using different mammalian cell types, we show that distinct higher order compartments whose DNA displays a specific replication timing are stably maintained during all interphase stages. The organizational principle is clonally inherited. We directly demonstrate the presence of polar chromosome territories that align to build up higher order compartments, as previously suggested (Ferreira et al., 1997). Polar chromosome territories display a specific orientation of early and late replicating subregions that correspond to R- or G/C-bands of mitotic chromosomes. Higher order compartments containing G/C-bands replicating during the second half of the S phase display no transcriptional activity detectable by BrUTP pulse labeling and show no evidence of transcriptional competence. Transcriptionally competent and active chromatin is confined to a coherent compartment within the nuclear interior that comprises early replicating R-band sequences. As a whole, the data provide an integrated view on chromosome structure, nuclear higher order compartmentalization, and their relation to the spatial organization of functional nuclear processes.
引用
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页码:1211 / 1226
页数:16
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