Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation

被引：14

作者：

Moon, MH

Kwon, H

Park, I

机构：

[1] Department of Chemistry, Kangnung National University, Kangnung

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1997年 / 20卷 / 16-17期

关键词：

D O I：

10.1080/10826079708005593

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.

引用

收藏

页码：2803 / 2814

页数：12

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