Mice deficient for a small cluster of Piwi-interacting RNAs implicate Piwi-interacting RNAs in transposon control

被引：61

作者：

Xu, Mingang ^{[1
]}

You, Yun ^{[2
]}

Hunsicker, Patricia ^{[2
]}

Hori, Tamaki ^{[1
]}

Small, Chris ^{[3
]}

Griswold, Michael D. ^{[3
]}

Hecht, Norman B. ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Ctr Res Reprod & Womens Hlth, Philadelphia, PA 19104 USA

[2] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37830 USA

[3] Washington State Univ, Dept Mol Biosci, Pullman, WA 99164 USA

来源：

BIOLOGY OF REPRODUCTION | 2008年 / 79卷 / 01期

关键词：

gametogenesis; gene regulation; male reproductive tract; piRNA; spermatogenesis; testis; transposon;

D O I：

10.1095/biolreprod.108.068072

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mammalian testis expresses a class of small noncoding RNAs that interact with mammalian PIWI proteins. In mice, the PIWI-interacting RNAs (piRNAs) partner with mammalian PIWI proteins, PIWIL1 and PIWIL2, also known as MIWI and MILI, to maintain transposon silencing in the germline genome. Here, we demonstrate that inactivation of Nct1/2, two noncoding RNAs encoding piRNAs, leads to derepression of LINE-1 (L1) but does not affect mouse viability, spermatogenesis, testicular gene expression, or fertility. These findings indicate that piRNAs from a cluster on chromosome 2 are necessary to maintain transposon silencing.

引用

页码：51 / 57

页数：7

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