Evaluation of multi-target and single-target liposomal drugs for the treatment of gastric cancer

被引：16

作者：

Chen, Chien-Ho ^{[2
]}

Liu, Der-Zen ^{[3
]}

Fang, Hsu-Wei ^{[4
]}

Liang, Hong-Jen ^{[5
]}

Yang, Tzu-Sheng ^{[4
]}

Lin, Shyr-Yi ^{[1
]}

机构：

[1] Taipei Med Univ & Hosp, Dept Internal Med & Primary Care Med, Taipei 110, Taiwan

[2] Taipei Med Univ, Sch Med Lab Sci & Biotechnol, Taipei 110, Taiwan

[3] Taipei Med Univ, Grad Inst Biomed Mat, Taipei 110, Taiwan

[4] Natl Taipei Univ Technol, Dept Chem Engn & Biotechnol, Taipei 106, Taiwan

[5] Yuanpei Univ, Dept Food Sci, Hsinchu 300, Taiwan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 06期

关键词：

liposome; octreotide; Arg-Gly-Asp (RGD); xenograft; gastric cancer;

D O I：

10.1271/bbb.80096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effects of multi- and single-target liposomal drugs on human gastric cancer cell AGS both in vitro and in vivo. The cytotoxic effect of dihydrotanshinone I was significantly enhanced by treatment with octreotide-polyethylene glycol(PEG)-liposome, Arg-Gly-Asp(RGD)-PEG-liposome, and RGD/octreotide-PEG-liposome encapsulated with 0.5 mu g/ml of dihydrotanshinone I to AGS cell for 24 h, compared to control. Furthermore, the AGS cell survival rate for multi-target versus single target liposomal drugs was significantly suppressed. Microsocpic examination revealed that significant cell death occurred in the multi- and single-target liposomal encapsulated drug groups. Significant suppression of tumor growth in AGS cell xenograft nude mice given octreotide-PEG-liposome, RGD/octreotide-PEG-liposome encapsulated drug, versus those given a free drug was noted after 13d of experimentation with the multi-targeted liposome: up to 60.75% and 41.2% reduction of tumor volume as compared to dimethylsulfoxide (DMSO) control and the free drug groups respectively. The treated animals showed no gross signs of toxicity. The results have potential clinical application.

引用

页码：1586 / 1594

页数：9

共 44 条

[1]

BAUER W, 1982, LIFE SCI, V31, P1133, DOI 10.1016/0024-3205(82)90087-X

[2] SPECIFIC TARGETING WITH POLY(ETHYLENE GLYCOL)-MODIFIED LIPOSOMES - COUPLING OF HOMING DEVICES TO THE ENDS OF THE POLYMERIC CHAINS COMBINES EFFECTIVE TARGET BINDING WITH LONG CIRCULATION TIMES [J].

BLUME, G ;

CEVC, G ;

CROMMELIN, MDJA ;

BAKKERWOUDENBERG, IAJM ;

KLUFT, C ;

STORM, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1149 (01) :180-184

[3] Role of integrins in angiogenesis [J].

Brooks, PC .

EUROPEAN JOURNAL OF CANCER, 1996, 32A (14) :2423-2429

[4] Tanshinone IIA from Salvia miltiorrhiza induces heme oxygenase-1 expression and inhibits lipopolysaccharide-induced nitric oxide expression in RAW 264.7 cells [J].

Chen, Tso-Hsiao ;

Hsu, Yu-Tern ;

Chen, Cheng-Hsien ;

Kao, Shu-Hwei ;

Lee, Horng-Mo .

MITOCHONDRION, 2007, 7 (1-2) :101-105

[5] Enhancing growth human endothelial cells on Arg-Gly-Asp (RGD) embedded poly (ε-caprolactone) (PCL) surface with nanometer scale of surface disturbance [J].

Chung, TW ;

Yang, MG ;

Liu, DZ ;

Chen, WP ;

Pan, CI ;

Wang, SS .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2005, 72A (02) :213-219

[6]

CLI F, 1998, CANCER, V83, P2475

[7]

Franek KJ, 2005, INT J ONCOL, V26, P217

[8]

GERDES J, 1984, J IMMUNOL, V133, P1710

[9] Liposome-encapsulated doxorubicin compared with conventional doxorubicin in a randomized multicenter trial as first-line therapy of metastatic breast carcinoma [J].

Harris, L ;

Batist, G ;

Belt, R ;

Rovira, D ;

Navari, R ;

Azarnia, N ;

Welles, K ;

Winer, E .

CANCER, 2002, 94 (01) :25-36

[10] Molecular mechanisms of econazole-induced toxicity on human colon cancer cells: G0/G1 cell cycle arrest and caspase 8-independent apoptotic signaling pathways [J].

Ho, YS ;

Wu, CH ;

Chou, HM ;

Wang, YJ ;

Tseng, H ;

Chen, CH ;

Chen, LC ;

Lee, CH ;

Lin, SY .

FOOD AND CHEMICAL TOXICOLOGY, 2005, 43 (10) :1483-1495

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