Autoaggregation of Lactobacillus reuteri is mediated by a putative DEAD-box helicase

被引:40
作者
Roos, S [1 ]
Lindgren, S [1 ]
Jonsson, H [1 ]
机构
[1] Swedish Univ Agr Sci, Dept Microbiol, S-75007 Uppsala, Sweden
关键词
D O I
10.1046/j.1365-2958.1999.01363.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned and sequenced a gene from Lactobacillus reuteri that encodes a 56 kDa protein, which mediates autoaggregation of the bacteria. Using an antiserum raised against extracellular proteins from the pig intestinal isolate L. reuteri 1063, we screened a genomic lambda library derived from the same strain, Affinity purification of recombinant protein from the isolated lambda clones showed that one type of clone expressed a protein that efficiently aggregated the parental strain when added to the bacteria, Subcloning and introduction of the corresponding gene, here denoted aggH, into the L. reuteritype strain markedly enhanced aggregation, Furthermore, insertional inactivation of aggH in strain 1063 resulted in an autoaggregation-deficient phenotype. Finally, an affinity-purified and cleaved fusion of AggH protein and the maltose-binding protein, MBP, strongly promoted aggregation of L, reuteri 1063, whereas the uncleaved fusion protein was inactive. Sequencing of aggH revealed that the corresponding protein has extensive sequence homology to the large family of ATP-dependent DEAD-box helicases. These results are intriguing in view of earlier data on the promotion of genetic exchange in Lactobacillus by aggregation.
引用
收藏
页码:427 / 436
页数:10
相关论文
共 35 条
[1]   TRANSFORMATION OF LACTOBACILLUS-REUTERI WITH ELECTROPORATION - STUDIES ON THE ERYTHROMYCIN RESISTANCE PLASMID PLUL631 [J].
AHRNE, S ;
MOLIN, G ;
AXELSSON, L .
CURRENT MICROBIOLOGY, 1992, 24 (04) :199-205
[2]   MOBILIZATION OF SMALL PLASMIDS IN BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS IS ACCOMPANIED BY SPECIFIC AGGREGATION [J].
ANDRUP, L ;
DAMGAARD, J ;
WASSERMANN, K .
JOURNAL OF BACTERIOLOGY, 1993, 175 (20) :6530-6536
[3]   CHARACTERIZATION AND DNA HOMOLOGY OF LACTOBACILLUS STRAINS ISOLATED FROM PIG INTESTINE [J].
AXELSSON, L ;
LINDGREN, S .
JOURNAL OF APPLIED BACTERIOLOGY, 1987, 62 (05) :433-440
[4]   IDENTIFICATION AND CLONING OF A PLASMID-ENCODED ERYTHROMYCIN RESISTANCE DETERMINANT FROM LACTOBACILLUS-REUTERI [J].
AXELSSON, LT ;
AHRNE, SEI ;
ANDERSSON, MC ;
STAHL, SR .
PLASMID, 1988, 20 (02) :171-174
[5]   Vaccinia virion protein I8R has both DNA and RNA helicase activities: Implications for vaccinia virus transcription [J].
Bayliss, CD ;
Smith, GL .
JOURNAL OF VIROLOGY, 1996, 70 (02) :794-800
[6]   CLONING AND MOLECULAR ANALYSIS OF GENES AFFECTING EXPRESSION OF BINDING SUBSTANCE, THE RECIPIENT-ENCODED RECEPTOR(S) MEDIATING MATING AGGREGATE FORMATION IN ENTEROCOCCUS-FAECALIS [J].
BENSING, BA ;
DUNNY, GM .
JOURNAL OF BACTERIOLOGY, 1993, 175 (22) :7421-7429
[7]   Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate [J].
Boris, S ;
Suarez, JE ;
Barbes, C .
JOURNAL OF APPLIED MICROBIOLOGY, 1997, 83 (04) :413-420
[8]  
Bos E S, 1981, J Immunoassay, V2, P187, DOI 10.1080/15321818108056977
[9]   MOLECULAR REARRANGEMENT OF LACTOSE PLASMID DNA ASSOCIATED WITH HIGH-FREQUENCY TRANSFER AND CELL-AGGREGATION IN LACTOCOCCUS-LACTIS 712 [J].
GASSON, MJ ;
SWINDELL, S ;
MAEDA, S ;
DODD, HM .
MOLECULAR MICROBIOLOGY, 1992, 6 (21) :3213-3223
[10]   HELICASES - AMINO-ACID-SEQUENCE COMPARISONS AND STRUCTURE-FUNCTION-RELATIONSHIPS [J].
GORBALENYA, AE ;
KOONIN, EV .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (03) :419-429