Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide of Porphyromonas gingivalis

被引：13

作者：

Shimauchi, H ^{[1
]}

Ogawa, T ^{[1
]}

Uchida, H ^{[1
]}

Yoshida, J ^{[1
]}

Ogoh, H ^{[1
]}

Nozaki, T ^{[1
]}

Okada, H ^{[1
]}

机构：

[1] OSAKA UNIV,FAC DENT,DEPT ORAL MICROBIOL,SUITA,OSAKA 565,JAPAN

来源：

EXPERIENTIA | 1996年 / 52卷 / 09期

关键词：

Porphyromonas gingivalis; lipopolysaccharide (LPS); C3H/HeJ mice; B-cells; protein phosphorylation; cytokine production;

D O I：

10.1007/BF01938879

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced by P. gingivalis LPS was incompletely inhibited by polymyxin B. P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared with Escherichia coli LPS. Furthermore, P. gingivalis LPS, but:not E. coli LPS, induced definite IL-6 production in C3H/HeJ mice. P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinated P. gingivalis LPS, similarly to E. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. Thus P. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells.

引用

页码：909 / 917

页数：9

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