Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

被引：144

作者：

Sato, Yasunori ^{[1
,2
]}

Kajiyama, Shizuo ^{[3
]}

Amano, Akiko ^{[2
]}

Kondo, Yoshitaka ^{[2
]}

Sasaki, Toru ^{[4
]}

Handa, Setsuko ^{[2
]}

Takahashi, Ryoya ^{[1
]}

Fukui, Michiaki ^{[5
]}

Hasegawa, Goji ^{[5
]}

Nakamura, Naoto ^{[5
]}

Fujinawa, Hikohito ^{[6
]}

Mori, Toyotaka ^{[6
]}

Ohta, Mitsuhiro ^{[7
]}

Obayashi, Hiroshi ^{[8
]}

Maruyama, Naoki ^{[2
]}

Ishigami, Akihito ^{[1
,2
]}

机构：

[1] Toho Univ, Fac Pharmaceut Sci, Dept Biochem, Chiba 2748510, Japan

[2] Tokyo Metropolitan Inst Gerontol, Aging Regulat, Tokyo 1730015, Japan

[3] Kajiyama Clin, Kyoto 6150035, Japan

[4] Tokyo Metropolitan Inst Gerontol, Res Team Mol Biomarker, Tokyo 1730015, Japan

[5] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Endocrinol & Metab, Kyoto 6028566, Japan

[6] Pharmaceut Co LTD, Tokyo 1410032, Japan

[7] Kobe Pharmaceut Univ, Dept Med Biochem, Kobe, Hyogo 6588566, Japan

[8] Inst Bio Response Informat, Kyoto 6128016, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 03期

关键词：

ascorbic acid; chemiluminescence; gluconolactonase; hydrogen-rich pure water; oxidative stress; ROS; senescence marker protein-30; superoxide; vitamin C;

D O I：

10.1016/j.bbrc.2008.08.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen is an established anti-oxidant that prevents acute oxidative Stress. To Clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice. which cannot synthesize vitamin C (VC) also a well-known anti-oxidant. These KO mice were divided into three groups: recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：346 / 350

页数：5

共 27 条

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