Transplantation of bone marrow-derived very small embryonic-like stem cells attenuates left ventricular dysfunction and remodeling after myocardial infarction

被引：109

作者：

Dawn, Buddhadeb ^{[1
]}

Tiwari, Sumit ^{[1
]}

Kucia, Magdalena J. ^{[2
]}

Zuba-Surma, Ewa K. ^{[2
]}

Guo, Yiru ^{[1
]}

SanganalMath, Santosh K. ^{[1
]}

Abdel-Latif, Ahmed ^{[1
]}

Hunt, Greg ^{[1
]}

Vincent, Robert J. ^{[1
]}

Taher, Hisham ^{[1
]}

Reed, Nathan J. ^{[1
]}

Ratajczak, Mariusz Z. ^{[2
]}

Bolli, Roberto ^{[1
]}

机构：

[1] Univ Louisville, Inst Mol Cardiol, Louisville, KY 40292 USA

[2] Univ Louisville, Stem Cell Biol Program, Louisville, KY 40292 USA

来源：

STEM CELLS | 2008年 / 26卷 / 06期

关键词：

myocardial infarction; myocardial regeneration; very small embryonic-like stem cell; stem cell; bone marrow; left ventricular function;

D O I：

10.1634/stemcells.2007-0715

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

a Adult bone marrow (BM) contains Sca-1+/Lin-/CD45- very small embryonic-like stem cells (VSELs) that express markers of several lineages, including cardiac markers, and differentiate into cardiomyocytes in vitro. We examined whether BM-derived VSELs promote myocardial repair after a reperfused myocardial infarction (MI). Mice underwent a 30-minute coronary occlusion followed by reperfusion and received intramyocardial injection of vehicle (n = 11), 1 x 10(5) Sca-1+/Lin-/CD45-enhanced green fluorescent protein (EGFP)-labeled hematopoietic stem cells (n = 13 [cell control group]), or 1 x 10(4) Sca-1+/Lin-/CD45- EGFP-labeled cells (n = 14 [VSEL-treated group]) at 48 hours after MI. At 35 days after MI, VSEL-treated mice exhibited improved global and regional left ventricular (LV) systolic function (echocardiography) and attenuated myocyte hypertrophy in surviving tissue (histology and echocardiography) compared with vehicle-treated controls. In contrast, transplantation of Sca-1+/Lin-/CD45- cells failed to confer any functional or structural benefits. Scattered EGFP+ myocytes and capillaries were present in the infarct region in VSEL-treated mice, but their numbers were very small. These results indicate that transplantation of a relatively small number of CD45+ VSELs is sufficient to improve LV function and alleviate myocyte hypertrophy after MI, supporting the potential therapeutic utility of these cells for cardiac repair.

引用

页码：1646 / 1655

页数：10

共 35 条

[1]

Adult bone marrow-derived cells for cardiac repair - A systematic review and meta-analysis [J].

Abdel-Latif, Ahmed ;

Bolli, Roberto ;

Tleyjeh, Imad M. ;

Montori, Victor M. ;

Perin, Emerson C. ;

Hornung, Carlton A. ;

Zuba-Surma, Ewa K. ;

Al-Mallah, Mouaz ;

Dawn, Buddhadeb .

ARCHIVES OF INTERNAL MEDICINE, 2007, 167 (10) :989-997

[2]

STRUCTURAL COMPENSATORY MECHANISMS IN RAT-HEART IN EARLY SPONTANEOUS HYPERTENSION [J].

ANVERSA, P ;

MELISSARI, M ;

BEGHI, C ;

OLIVETTI, G .

AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 246 (06) :H739-H746

[3]

MYOCARDIAL-INFARCTION IN RATS - INFARCT SIZE, MYOCYTE HYPERTROPHY, AND CAPILLARY GROWTH [J].

ANVERSA, P ;

BEGHI, C ;

KIKKAWA, Y ;

OLIVETTI, G .

CIRCULATION RESEARCH, 1986, 58 (01) :26-37

[4]

Anversa P., 2002, HDB PHYSL CARDIOVASC, VI, P75, DOI DOI 10.1002/CPHY.CP020102

[5]

Adult cardiac stem cells are multipotent and support myocardial regeneration [J].

Beltrami, AP ;

Barlucchi, L ;

Torella, D ;

Baker, M ;

Limana, F ;

Chimenti, S ;

Kasahara, H ;

Rota, M ;

Musso, E ;

Urbanek, K ;

Leri, A ;

Kajstura, J ;

Nadal-Ginard, B ;

Anversa, P .

CELL, 2003, 114 (06) :763-776

[6]

Bone marrow cell-mediated cardiac regeneration - A veritable revolution [J].

Bolli, R ;

Jneid, H ;

Dawn, B .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2005, 46 (09) :1659-1661

[7]

Postinfarct cytokine therapy regenerates cardiac tissue and improves left ventricular function [J].

Dawn, B ;

Guo, YR ;

Rezazadeh, A ;

Huang, YM ;

Stein, AB ;

Hunt, G ;

Tiwari, S ;

Varma, J ;

Gu, Y ;

Prabhu, SD ;

Kajstura, J ;

Anversa, P ;

Ildstad, ST ;

Bolli, R .

CIRCULATION RESEARCH, 2006, 98 (08) :1098-1105

[8]

Cardiac stem cells delivered intravascularly traverse the vessel barrier, regenerate infarcted myocardium, and improve cardiac function [J].

Dawn, B ;

Stein, AB ;

Urbanek, K ;

Rota, M ;

Whang, B ;

Rastaldo, R ;

Torella, D ;

Tang, XL ;

Rezazadeh, A ;

Kajstura, J ;

Leri, A ;

Hunt, G ;

Varma, J ;

Prabhu, SD ;

Anversa, P ;

Bolli, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (10) :3766-3771

[9]

Adult bone marrow-derived cells: Regenerative potential, plasticity, and tissue commitment [J].

Dawn, B ;

Bolli, R .

BASIC RESEARCH IN CARDIOLOGY, 2005, 100 (06) :494-503

[10]

The late phase of ischemic preconditioning is abrogated by targeted disruption of the inducible NO synthase gene [J].

Guo, Y ;

Jones, WK ;

Xuan, YT ;

Tang, XL ;

Bao, W ;

Wu, WJ ;

Han, H ;

Laubach, VE ;

Ping, PP ;

Yang, ZQ ;

Qiu, YM ;

Bolli, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11507-11512

← 1 2 3 4 →