Single-molecule detection of coumarin-120

被引：6

作者：

Brand, L ^{[1
]}

Eggeling, C ^{[1
]}

Seidel, CAM ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,D-37077 GOTTINGEN,GERMANY

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 5-6期

关键词：

D O I：

10.1080/07328319708002915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resulting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasenstive DNA characterization.

引用

页码：551 / 556

页数：6

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