共 54 条
Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web
被引:116
作者:

Espenel, Cedric
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France

Margeat, Emmanuel
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France

Dosset, Patrice
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France

Arduise, Cecile
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U602, F-94804 Villejuif, France
Univ Paris 11, Inst Andre Lwoff, F-94801 Villejuif, France INSERM, U554, F-34090 Montpellier, France

Le Grimellec, Christian
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France

Royer, Catherine A.
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France

Boucheix, Claude
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U602, F-94804 Villejuif, France
Univ Paris 11, Inst Andre Lwoff, F-94801 Villejuif, France INSERM, U554, F-34090 Montpellier, France

Rubinstein, Eric
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U602, F-94804 Villejuif, France
Univ Paris 11, Inst Andre Lwoff, F-94801 Villejuif, France INSERM, U554, F-34090 Montpellier, France

Milhiet, Pierre-Emmanuel
论文数: 0 引用数: 0
h-index: 0
机构:
INSERM, U554, F-34090 Montpellier, France
Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France INSERM, U554, F-34090 Montpellier, France
机构:
[1] INSERM, U554, F-34090 Montpellier, France
[2] Univ Montpellier, CNRS, Unite Mixte Rech 5048, Ctr Biochim Struct, F-34090 Montpellier, France
[3] INSERM, U602, F-94804 Villejuif, France
[4] Univ Paris 11, Inst Andre Lwoff, F-94801 Villejuif, France
关键词:
D O I:
10.1083/jcb.200803010
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Tetraspanins regulate cell migration, sperm-egg fusion, and viral infection. Through interactions with one another and other cell surface proteins, tetraspanins form a network of molecular interactions called the tetraspanin web. In this study, we use single-molecule fluorescence microscopy to dissect dynamics and partitioning of the tetraspanin CD9. We show that lateral mobility of CD9 in the plasma membrane is regulated by at least two modes of interaction that each exhibit specific dynamics. The majority of CD9 molecules display Brownian behavior but can be transiently confined to an interaction platform that is in permanent exchange with the rest of the membrane. These platforms, which are enriched in CD9 and its binding partners, are constant in shape and localization. Two CD9 molecules undergoing Brownian trajectories can also codiffuse, revealing extra platform interactions. CD9 mobility and partitioning are both dependent on its palmitoylation and plasma membrane cholesterol. Our data show the high dynamic of interactions in the tetraspanin web and further indicate that the tetraspanin web is distinct from raft microdomains.
引用
收藏
页码:765 / 776
页数:12
相关论文
共 54 条
[1]
Expression of the palmitoylation-deficient CD151 weakens the association of α3β1 integrin with the tetraspanin-enriched microdomains and affects integrin-dependent signaling
[J].
Berditchevski, F
;
Odintsova, E
;
Sawada, S
;
Gilbert, E
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2002, 277 (40)
:36991-37000

Berditchevski, F
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England

Odintsova, E
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England

Sawada, S
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England

Gilbert, E
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England Univ Birmingham, Canc Res UK, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England
[2]
Tetraspanins
[J].
Boucheix, C
;
Rubinstein, E
.
CELLULAR AND MOLECULAR LIFE SCIENCES,
2001, 58 (09)
:1189-1205

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构:
Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构:
Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France
[3]
A physical and functional link between cholesterol and tetraspanins
[J].
Charrin, S
;
Manié, S
;
Thiele, C
;
Billard, M
;
Gerlier, D
;
Boucheix, C
;
Rubinstein, E
.
EUROPEAN JOURNAL OF IMMUNOLOGY,
2003, 33 (09)
:2479-2489

论文数: 引用数:
h-index:
机构:

Manié, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France

Thiele, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France

Billard, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France

Gerlier, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 11, Inst Andre Lwoff, INSERM, U268, Villejuif, France
[4]
EWI-2 is a new component of the tetraspanin web in hepatocytes and lymphoid cells
[J].
Charrin, S
;
Le Naour, F
;
Labas, V
;
Billard, M
;
Le Caer, JP
;
Emile, JF
;
Petit, MA
;
Boucheix, C
;
Rubinstein, E
.
BIOCHEMICAL JOURNAL,
2003, 373
:409-421

Charrin, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Le Naour, F
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Labas, V
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Billard, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Le Caer, JP
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Emile, JF
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Petit, MA
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM U268, F-94807 Villejuif, France
[5]
Multiple levels of interactions within the tetraspanin web
[J].
Charrin, S
;
Manié, S
;
Billard, M
;
Ashman, L
;
Gerlier, D
;
Boucheix, C
;
Rubinstein, E
.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
2003, 304 (01)
:107-112

Charrin, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Manié, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Billard, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Ashman, L
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Gerlier, D
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构:
Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France
[6]
Differential stability of tetraspanin/tetraspanin interactions:: role of palmitoylation
[J].
Charrin, S
;
Manié, S
;
Oualid, M
;
Billard, M
;
Boucheix, C
;
Rubinstein, E
.
FEBS LETTERS,
2002, 516 (1-3)
:139-144

Charrin, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France

Manié, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France

Oualid, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France

Billard, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构:
Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France
[7]
The major CD9 and CD81 molecular partner - Identification and characterization of the complexes
[J].
Charrin, S
;
Le Naour, F
;
Oualid, M
;
Billard, M
;
Faure, G
;
Hanash, SM
;
Boucheix, C
;
Rubinstein, E
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2001, 276 (17)
:14329-14337

Charrin, S
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Le Naour, F
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Oualid, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Billard, M
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Faure, G
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Hanash, SM
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Boucheix, C
论文数: 0 引用数: 0
h-index: 0
机构: Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构:
Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France Hop Paul Brousse, INSERM, U268, F-94807 Villejuif, France
[8]
Transient anchorage of cross-linked glycosyl-phosphatidylinositol-anchored proteins depends on cholesterol, Src family kinases, caveolin, and phosphoinositides
[J].
Chen, Yun
;
Thelin, William R.
;
Yang, Bing
;
Milgram, Sharon L.
;
Jacobson, Ken
.
JOURNAL OF CELL BIOLOGY,
2006, 175 (01)
:169-178

Chen, Yun
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

Thelin, William R.
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

Yang, Bing
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

Milgram, Sharon L.
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

Jacobson, Ken
论文数: 0 引用数: 0
h-index: 0
机构:
Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA Univ N Carolina, Sch Med, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
[9]
Hepatitis C virus entry: potential receptors and their biological functions
[J].
Cocquerel, L
;
Voisset, C
;
Dubuisson, J
.
JOURNAL OF GENERAL VIROLOGY,
2006, 87
:1075-1084

Cocquerel, L
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France

Voisset, C
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France

Dubuisson, J
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France Inst Pasteur, Inst Biol Lille, CNRS UMR 8161, F-59021 Lille, France
[10]
Tetraspanin CD82 controls the association of cholesterol-dependent microdomains with the actin cytoskeleton in T lymphocytes: relevance to co-stimulation
[J].
Delaguillaumie, A
;
Harriague, J
;
Kohanna, S
;
Bismuth, G
;
Rubinstein, E
;
Seigneuret, M
;
Conjeaud, H
.
JOURNAL OF CELL SCIENCE,
2004, 117 (22)
:5269-5282

Delaguillaumie, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Harriague, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Kohanna, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Bismuth, G
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Rubinstein, E
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Seigneuret, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France

Conjeaud, H
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 05, Dept Biol Cellulaire, Inst Cochin, INSERM,U567,CNRS,UMR 8104, F-75014 Paris, France