Optimal cytokine stimulation for the enhanced generation of leukemic dendritic cells in short-term culture

被引：28

作者：

Panoskaltsis, N ^{[1
]}

Belanger, TJ ^{[1
]}

Liesveld, JL ^{[1
]}

Abboud, CN ^{[1
]}

机构：

[1] Univ Rochester, Med Ctr, Dept Internal Med, Hematol Oncol Unit, Rochester, NY 14642 USA

来源：

LEUKEMIA RESEARCH | 2002年 / 26卷 / 02期

关键词：

leukemia; dendritic cell; immunotherapy; cytokine; AML; culture;

D O I：

10.1016/S0145-2126(01)00104-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Dendritic cells (DCs) are professional antigen presenting cells derived from myeloid or lymphoid precursors. Functional DCs have been generated from the malignant counterpart of these precursor cells. Herein, we describe the generation of DCs from different leukemias and determine the optimal culture conditions with minimal manipulation. Primary leukemic cells were cultured for 1, 3, and 5 days in 11 different cytokine combinations and analyzed for the expression of a mature DC phenotype. Optimal growth and DC characteristics were obtained with GM-CSF, FL, and SCF in 3-5 day cultures, suggesting a practical strategy for the immunotherapy of leukemia. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：191 / 201

页数：11

共 41 条

[1] Dendritic cells and the control of immunity [J].

Banchereau, J ;

Steinman, RM .

NATURE, 1998, 392 (6673) :245-252

[2] Mechanisms of the graft-versus-leukemia reaction [J].

Barrett, AJ .

STEM CELLS, 1997, 15 (04) :248-258

[3] Help for cytotoxic-T-cell responses is mediated by CD40 signalling [J].

Bennett, SRM ;

Carbone, FR ;

Karamalis, F ;

Flavell, RA ;

Miller, JFAP ;

Heath, WR .

NATURE, 1998, 393 (6684) :478-480

[4] Generation of functional human dendritic cells from adherent peripheral blood monocytes by CD40 ligation in the absence of granulocyte-macrophage colony-stimulating factor [J].

Brossart, P ;

Grünebach, F ;

Stuhler, G ;

Reichardt, VL ;

Möhle, R ;

Kanz, L ;

Brugger, WR .

BLOOD, 1998, 92 (11) :4238-4247

[5] Ex vivo generation of human anti-pre-B leukemia-specific autologous cytolytic T cells [J].

Cardoso, AA ;

Seamon, MJ ;

Afonso, HM ;

Ghia, P ;

Boussiotis, VA ;

Freeman, GJ ;

Gribben, JG ;

Sallan, SE ;

Nadler, LM .

BLOOD, 1997, 90 (02) :549-561

[6]

Charbonnier A, 1999, EUR J IMMUNOL, V29, P2567, DOI 10.1002/(SICI)1521-4141(199908)29:08<2567::AID-IMMU2567>3.0.CO

[7]

2-S

[8] Dendritic cells derived in vitro from acute myelogenous leukemia cells stimulate autologous, antileukemic T-cell responses [J].

Choudhury, A ;

Liang, JC ;

Thomas, EK ;

Flores-Romo, L ;

Xie, QS ;

Agusala, K ;

Sutaria, S ;

Sinha, I ;

Champlin, RE ;

Claxton, DF .

BLOOD, 1999, 93 (03) :780-786

[9] Human leukemia-derived dendritic cells: Ex-vivo development of specific antileukemic cytotoxicity [J].

Choudhury, A ;

Toubert, A ;

Sutaria, S ;

Charron, D ;

Champlin, RE ;

Claxton, DF .

CRITICAL REVIEWS IN IMMUNOLOGY, 1998, 18 (1-2) :121-131

[10] Use of leukemic dendritic cells for the generation of antileukemic cellular cytotoxicity against Philadelphia chromosome-positive chronic myelogenous leukemia [J].

Choudhury, A ;

Gajewski, JL ;

Liang, JC ;

Popat, U ;

Claxton, DF ;

Kliche, KO ;

Andreeff, M ;

Champlin, RE .

BLOOD, 1997, 89 (04) :1133-1142

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