The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains

被引:29
作者
Opel, KL
Chung, DT
Drábek, J
Tatarek, NE
Jantz, LM
McCord, BR
机构
[1] Florida Int Univ, Dept Chem & Biochem, Miami, FL 33199 USA
[2] Ohio Univ, Dept Chem & Biochem, Clippinger Labs 136, Athens, OH 45701 USA
[3] Ohio Univ, Dept Sociol & Anthropol, Athens, OH 45701 USA
[4] Univ Tennessee, Dept Anthropol, Knoxville, TN 37996 USA
关键词
forensic science; multiplex PCR; short tandem repeat; STR; nuclear DNA; DNA typing; human skeletal remains;
D O I
10.1111/j.1556-4029.2006.00077.x
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex(T) 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex((R)) 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.
引用
收藏
页码:351 / 356
页数:6
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