Lack of hotspot targets:: a constraint for IS30 transposition in Salmonella

被引:6
作者
Casadesús, J [1 ]
Naas, T
Garzón, A
Arini, A
Torreblanca, J
Arber, W
机构
[1] Univ Sevilla, Dept Genet, Fac Biol, E-41080 Seville, Spain
[2] Univ Basel, Abt Mikrobiol, Biozentrum, CH-4056 Basel, Switzerland
关键词
bacterial genome; DNA composition; horizontal transfer; insertion elements;
D O I
10.1016/S0378-1119(99)00256-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
IS30 is an insertion element common in E. coli strains but rare or absent in Salmonella. Transfer of the IS30-flanked transposon Tn2700 to Salmonella typhimurium was assayed using standard delivery procedures of bacterial genetics (conjugation and transduction). Tn2700 'hops' were rare and required transposase overproduction, suggesting the existence of host constraints for IS30 activity. Sequencing of three Tn2700 insertions in the genome of S. typhimurium revealed that the transposon had been inserted into sites with a low homology to the IS30 consensus target, suggesting that inefficient Tn2700 transposition to the Salmonella genome might be caused by a lack of hotspot targets. This view was confirmed by the introduction of an IS30 'hot target sequence', whose sole presence permitted Tn2700 transposition without transposase overproduction. Detection of IS30-induced DNA rearrangements in S. typhimurium provided further evidence that the element undergoes similar activities in E. coli and S. typhimurium. Thus, hotspot absence may be the main (if not the only) limitation for IS30 activity in the latter species. If these observations faithfully reproduce the scenario of natural populations, establishment of IS30 in the Salmonella genome may have been prevented by a lack of DNA sequences closely related to the unusually long (24 bp) IS30 consensus target. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:231 / 239
页数:9
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