Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

被引:920
作者
Nekrasov, Vladimir [1 ]
Staskawicz, Brian [2 ]
Weigel, Detlef [3 ]
Jones, Jonathan D. G. [1 ]
Kamoun, Sophien [1 ]
机构
[1] Norwich Res Pk, Sainsbury Lab, Norwich, Norfolk, England
[2] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[3] Max Planck Inst Dev Biol, Tubingen, Germany
基金
英国生物技术与生命科学研究理事会;
关键词
We thank F. Ausubel for critical reading of the article; D. Voytas for discussion on the HDR strategy; Y. Xiong for the CYCD3 expression plasmid. J.F.L. is supported by the MGH ECOR Postdoctoral Fellowship for Medical Discovery. The Research is supported by the Department of Energy grant DE-FG02-02ER63445 to G.M.C; the National Science Foundation grant IOS-0843244 and the National Institutes of Health grants R01 GM60493 and R01 GM70567 to J.S.We thank S. Marillonnet and Icon Genetics; Halle; Germany for providing plasmid vectors; J. Win and S. Dong for help with figure preparation; and M. Smoker for help with the plant transformation. This work was supported by the Gatsby Charitable Foundation; the European Research Council (ERC); and the Biotechnology and Biological Sciences Research Council (BBSRC);
D O I
10.1038/nbt.2655
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
9
引用
收藏
页码:691 / 693
页数:4
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