Identification of genes coding for tumor antigens recognized by cytolytic T lymphocytes

被引：59

作者：

DePlaen, E ^{[1
]}

Lurquin, C ^{[1
]}

Lethe, B ^{[1
]}

vanderBruggen, P ^{[1
]}

Brichard, V ^{[1
]}

Renauld, JC ^{[1
]}

Coulie, P ^{[1
]}

VanPel, A ^{[1
]}

Boon, T ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN, CELL GENET UNIT, B-1200 BRUSSELS, BELGIUM

来源：

METHODS | 1997年 / 12卷 / 02期

关键词：

D O I：

10.1006/meth.1997.0462

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Strategies have been developed to characterize tumor antigens recognized by cytolytic T lymphocytes (CTL). We use a genetic approach based on the transfection of HLA genes and cDNA libraries in COS cells to isolate the gene producing the antigenic peptide. The tumor-specific expression of this gene can be evaluated by cDNA synthesis and quantitative PCR amplification. Transfection of fragments of the isolated gene allows the identification of the region encoding the antigenic peptide. Peptides are synthesized and tested for their ability to sensitize target cells to lysis by the CTL. (C) 1997 Academic Press.

引用

页码：125 / 142

页数：18

共 85 条

[1] ISOLATION AND EXPRESSION OF FUNCTIONAL HIGH-AFFINITY FC RECEPTOR COMPLEMENTARY DNAS [J].

ALLEN, JM ;

SEED, B .

SCIENCE, 1989, 243 (4889) :378-381

[2] CLONAL ANALYSIS OF THE CYTOLYTIC T-CELL RESPONSE TO HUMAN-TUMORS [J].

ANICHINI, A ;

FOSSATI, G ;

PARMIANI, G .

IMMUNOLOGY TODAY, 1987, 8 (12) :385-389

[3] MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM [J].

ARUFFO, A ;

SEED, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8573-8577

[4]

Ausubel FM., 1993, Current Protocols in Molecular Biology

[5] PRODUCTION OF MONOCLONAL ANTIBODIES TO GROUP-A ERYTHROCYTES, HLA AND OTHER HUMAN CELL-SURFACE ANTIGENS - NEW TOOLS FOR GENETIC-ANALYSIS [J].

BARNSTABLE, CJ ;

BODMER, WF ;

BROWN, G ;

GALFRE, G ;

MILSTEIN, C ;

WILLIAMS, AF ;

ZIEGLER, A .

CELL, 1978, 14 (01) :9-20

[6] CONSTRUCTION OF A FUSION GENE THAT CONFERS RESISTANCE AGAINST HYGROMYCIN-B TO MAMMALIAN-CELLS IN CULTURE [J].

BERNARD, HU ;

KRAMMER, G ;

ROWEKAMP, WG .

EXPERIMENTAL CELL RESEARCH, 1985, 158 (01) :237-243

[7] LITHIUM-CHLORIDE POTENTIATES TUMOR NECROSIS FACTOR-MEDIATED CYTO-TOXICITY INVITRO AND INVIVO [J].

BEYAERT, R ;

VANHAESEBROECK, B ;

SUFFYS, P ;

VANROY, F ;

FIERS, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9494-9498

[8] THE FOREIGN ANTIGEN-BINDING SITE AND T-CELL RECOGNITION REGIONS OF CLASS-I HISTOCOMPATIBILITY ANTIGENS [J].

BJORKMAN, PJ ;

SAPER, MA ;

SAMRAOUI, B ;

BENNETT, WS ;

STROMINGER, JL ;

WILEY, DC .

NATURE, 1987, 329 (6139) :512-518

[9] ANTI-HLA-A2 ANTIBODY-ENHANCEMENT OF PEPTIDE ASSOCIATION WITH HLA-A2 AS DETECTED BY CYTO-TOXIC LYMPHOCYTES-T [J].

BODMER, H ;

OGG, G ;

GOTCH, F ;

MCMICHAEL, A .

NATURE, 1989, 342 (6248) :443-446

[10] BAGE - A NEW GENE ENCODING AN ANTIGEN RECOGNIZED ON HUMAN MELANOMAS BY CYTOLYTIC T-LYMPHOCYTES [J].

BOEL, P ;

WILDMANN, C ;

SENSI, ML ;

BRASSEUR, R ;

RENAULD, JC ;

COULIE, P ;

BOON, T ;

VANDERBRUGGEN, P .

IMMUNITY, 1995, 2 (02) :167-175

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