A simple and rapid technique for detecting protein phosphorylation using one-dimensional isoelectric focusing gels and immunoblot analysis

被引：19

作者：

Anderson, Jeffrey C. ^{[1
]}

Peck, Scott C. ^{[1
]}

机构：

[1] Univ Missouri, Dept Biochem, Columbia, MO 65201 USA

来源：

PLANT JOURNAL | 2008年 / 55卷 / 05期

关键词：

phosphorylation; isoelectric focusing; Arabidopsis; signal transduction;

D O I：

10.1111/j.1365-313X.2008.03550.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We report a technique for detecting protein phosphorylation that involves isoelectric focusing in a vertical mini-gel format followed by immunoblot detection of the target protein. This method uses standard protein gel equipment, allows sensitive detection of protein phosphorylation when phosphospecific antibodies are not available, and provides a stoichiometric measure of phosphorylation. We demonstrate the application of this method for observing phosphorylation of an Arabidopsis thaliana protein in response to biotic stress.

引用

页码：881 / 885

页数：5

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