IL-1 acts on antigen-presenting cells to enhance the in vivo proliferation of antigen-stimulated naive CD4 T cells via a CD28-dependent mechanism that does not involve increased expression of CD28 ligands

被引:33
作者
Khoruts, A
Osness, RE
Jenkins, MK
机构
[1] Univ Minnesota, Sch Med, Ctr Immunol, Dept Microbiol,MMC 334, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Sch Med, Ctr Immunol, Dept Med, Minneapolis, MN 55455 USA
关键词
adjuvant; IL-1; CD28;
D O I
10.1002/eji.200324170
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The basis of the adjuvant effect of IL-1 on the clonal expansion of Ag-specific CD4 T cells was measured in vivo using the TCR transgenic T cell adoptive transfer method. Injection of Ag plus IL-1 caused naive CD4 T cells to proliferate to a greater extent than did injection of Ag alone. The T cells had to express CD28 to experience the beneficial effect of IL-1 whereas the host had to express the type I IL-1 R, raising the possibility that IL-1's adjuvant properties were related to induction of CD28 ligands on APC. Surprisingly, however, expression of CD28 ligands on DC and B cells was not affected by IL-1. Therefore, the adjuvant properties of IL-1 depend on a basal level of CD28 signaling but cannot be explained by enhanced CD28 signaling due to CD28 ligand induction.
引用
收藏
页码:1085 / 1090
页数:6
相关论文
共 29 条
  • [1] Immunological memory and protective immunity: Understanding their relation
    Ahmed, R
    Gray, D
    [J]. SCIENCE, 1996, 272 (5258) : 54 - 60
  • [2] Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis
    Akiba, H
    Miyahira, Y
    Atsuta, M
    Takeda, K
    Nohara, C
    Futagawa, T
    Matsuda, H
    Aoki, T
    Yagita, H
    Okumura, K
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (02) : 375 - 380
  • [3] Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements
    Barnden, MJ
    Allison, J
    Heath, WR
    Carbone, FR
    [J]. IMMUNOLOGY AND CELL BIOLOGY, 1998, 76 (01) : 34 - 40
  • [4] Brocker T, 1999, EUR J IMMUNOL, V29, P1610, DOI 10.1002/(SICI)1521-4141(199905)29:05<1610::AID-IMMU1610>3.0.CO
  • [5] 2-8
  • [6] Curtsinger JM, 1999, J IMMUNOL, V162, P3256
  • [7] Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo
    DeSmedt, T
    Pajak, B
    Muraille, E
    Lespagnard, L
    Heinen, E
    DeBaetselier, P
    Urbain, J
    Leo, O
    Moser, M
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (04) : 1413 - 1424
  • [8] Visualization of specific B and T lymphocyte interactions in the lymph node
    Garside, P
    Ingulli, E
    Merica, RR
    Johnson, JG
    Noelle, RJ
    Jenkins, MK
    [J]. SCIENCE, 1998, 281 (5373) : 96 - 99
  • [9] Glaccum MB, 1997, J IMMUNOL, V159, P3364
  • [10] THE MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED ANTIGEN RECEPTOR ON T-CELLS .1. ISOLATION WITH A MONOCLONAL-ANTIBODY
    HASKINS, K
    KUBO, R
    WHITE, J
    PIGEON, M
    KAPPLER, J
    MARRACK, P
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1983, 157 (04) : 1149 - 1169