DNA restriction patterns produced by pulsed-field gel electrophoresis in Moraxella catarrhalis isolated from different geographical areas

被引：9

作者：

Martinez, G ^{[1
]}

Ahmed, K ^{[1
]}

Zheng, CH ^{[1
]}

Watanabe, K ^{[1
]}

Oishi, K ^{[1
]}

Nagatake, T ^{[1
]}

机构：

[1] Nagasaki Univ, Dept Internal Med, Inst Trop Med, Nagasaki 8528102, Japan

来源：

EPIDEMIOLOGY AND INFECTION | 1999年 / 122卷 / 03期

关键词：

D O I：

10.1017/S0950268899002381

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Pulsed field gel electrophoresis (PFGE) of the genomic DNA of Moraxella catarrhalis was done in 172 strains isolated from sputum of patients with respiratory infections in Nagasaki (130 strains), Europe (14 strains), Thailand (6 strains), Uganda (3 strains), Bangladesh (5 strains) and Kuwait (14 strains). Restriction endonuclease with SmaI generated 4-16 DNA fragments ranging from 1000 kb to 24.25 kb and was classified into 31 major groups. It was found that there were wide variations of DNA restriction patterns of strains isolated from the same and different geographical areas. DNA restriction patterns of strains isolated in Nagasaki during the last 12 years showed dynamic changes of the predominant strains in each time period. We conclude from this study that PFGE is a suitable method to document interstrain variation in M. catarrhalis.

引用

页码：417 / 422

页数：6

共 17 条

[1] FIMBRIATION, HEMAGGLUTINATION AND ADHERENCE PROPERTIES OF FRESH CLINICAL ISOLATES OF BRANHAMELLA-CATARRHALIS [J].

AHMED, K ;

RIKITOMI, N ;

MATSUMOTO, K .

MICROBIOLOGY AND IMMUNOLOGY, 1992, 36 (10) :1009-1017

[2]

AHMED K, 1999, UNPUB QUANTITATIVE Q

[3]

BEALIEU D, 1993, J CLIN MICROBIOL, V31, P736

[4]

Carter G L, 1994, Prog Ind Microbiol, V29, P667

[5] BRANHAMELLA-CATARRHALIS - PHENOTYPIC CHARACTERISTICS [J].

DOERN, GV .

AMERICAN JOURNAL OF MEDICINE, 1990, 88 (5A) :S33-S35

[6] CONSTRUCTION OF A COMBINED NOTI/SMAI PHYSICAL AND GENETIC-MAP OF MORAXELLA (BRANHAMELLA) CATARRHALIS STRAIN ATCC25238 [J].

FURIHATA, K ;

SATO, K ;

MATSUMOTO, H .

MICROBIOLOGY AND IMMUNOLOGY, 1995, 39 (10) :745-751

[7]

ISENBERG HD, 1998, ESSENTIAL PROCEDURES, P54

[8] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) OF GENOMIC DNA OF MORAXELLA (BRANHAMELLA) CATARRHALIS ISOLATES IN A HOSPITAL [J].

KAWAKAMI, Y ;

UENO, I ;

KATSUYAMA, T ;

FURIHATA, K ;

MATSUMOTO, H .

MICROBIOLOGY AND IMMUNOLOGY, 1994, 38 (11) :891-895

[9] USE OF SECONDARY PULSED-FIELD GEL-ELECTROPHORESIS IN SEPARATION OF LARGE DNA [J].

LAI, E ;

BIRREN, BW .

ANALYTICAL BIOCHEMISTRY, 1995, 224 (01) :68-74

[10] SURFACE-EXPOSED AND ANTIGENICALLY CONSERVED DETERMINANTS OF OUTER-MEMBRANE PROTEINS OF BRANHAMELLA-CATARRHALIS [J].

MURPHY, TF ;

BARTOS, LC .

INFECTION AND IMMUNITY, 1989, 57 (10) :2938-2941

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