Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation

被引：45

作者：

Cha, SK ^{[1
]}

Kim, NH ^{[1
]}

Lee, SM ^{[1
]}

Baik, CS ^{[1
]}

Lee, HT ^{[1
]}

Chung, KS ^{[1
]}

机构：

[1] KONKUK UNIV, ANIM RESOURCES RES CTR, KWANGJIN GU, SEOUL 143701, SOUTH KOREA

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 1997年 / 9卷 / 04期

关键词：

pig; parthenogenesis; ploidy;

D O I：

10.1071/R96078

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P < 0.05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote.

引用

页码：441 / 446

页数：6

共 34 条

[1] THE INDUCTION OF REVERSIBLE AND IRREVERSIBLE CHROMOSOME DECONDENSATION BY PROTEIN-SYNTHESIS INHIBITION DURING MEIOTIC MATURATION OF MOUSE OOCYTES [J].

CLARKE, HJ ;

MASUI, Y .

DEVELOPMENTAL BIOLOGY, 1983, 97 (02) :291-301

[2] FACTORS AFFECTING THE EFFICIENCY OF NUCLEAR TRANSPLANTATION IN THE RABBIT EMBRYO [J].

COLLAS, P ;

ROBL, JM .

BIOLOGY OF REPRODUCTION, 1990, 43 (05) :877-884

[3] THE XENOPUS CDC2 PROTEIN IS A COMPONENT OF MPF, A CYTOPLASMIC REGULATOR OF MITOSIS [J].

DUNPHY, WG ;

BRIZUELA, L ;

BEACH, D ;

NEWPORT, J .

CELL, 1988, 54 (03) :423-431

[4] COLCHICINE-INDUCED HETEROPLOIDY IN THE MOUSE .2. THE INDUCTION OF TETRAPLOIDY AND OTHER TYPES OF HETEROPLOIDY [J].

EDWARDS, RG .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1958, 137 (02) :349-362

[5] PARTHENOGENETIC DEVELOPMENT OF BOVINE OOCYTES TREATED WITH ETHANOL AND CYTOCHALASIN-B AFTER INVITRO MATURATION [J].

FUKUI, Y ;

SAWAI, K ;

FURUDATE, M ;

SATO, N ;

IWAZUMI, Y ;

OHSAKI, K .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1992, 33 (03) :357-362

[6] IN-VITRO DEVELOPMENT OF IN VITRO-MATURED PORCINE OOCYTES FOLLOWING CHEMICAL ACTIVATION OR IN-VITRO FERTILIZATION [J].

FUNAHASHI, H ;

CANTLEY, TC ;

STUMPF, TT ;

TERLOUW, SL ;

DAY, BN .

BIOLOGY OF REPRODUCTION, 1994, 50 (05) :1072-1077

[7] DEVELOPMENTAL ABILITY OF PORCINE OOCYTES MATURED AND FERTILIZED IN-VITRO [J].

FUNAHASHI, H ;

STUMPF, TT ;

TERLOUW, SL ;

CANTLEY, TC ;

RIEKE, A ;

DAY, BN .

THERIOGENOLOGY, 1994, 41 (07) :1425-1433

[8] Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes [J].

Funahashi, H ;

Kim, NH ;

Stumpf, TT ;

Cantley, TC ;

Day, BN .

BIOLOGY OF REPRODUCTION, 1996, 54 (06) :1412-1419

[9] ACTIVATION OF MURINE OOCYTES WITH CA2+ IONOPHORE AND CYCLOHEXIMIDE [J].

HAGEMANN, LJ ;

HILLERYWEINHOLD, FL ;

RUTLEDGE, MLL ;

FIRST, NL .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1995, 271 (01) :57-61

[10] RESPONSE OF PORCINE OOCYTES TO ELECTRICAL AND CHEMICAL ACTIVATION DURING MATURATION INVITRO [J].

HAGEN, DR ;

PRATHER, RS ;

FIRST, NL .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 28 (01) :70-73

← 1 2 3 4 →