Absorption and fluorescence spectroscopic investigation of indocyanine green

被引:231
作者
Philip, R
Penzkofer, A
Baumler, W
Szeimies, RM
Abels, C
机构
[1] UNIV REGENSBURG, DEPT PHYS, D-93040 REGENSBURG, GERMANY
[2] UNIV REGENSBURG, DEPT DERMATOL, D-93040 REGENSBURG, GERMANY
关键词
absorption; fluorescence; indocyanine green;
D O I
10.1016/1010-6030(95)04292-X
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Absorption spectra, fluorescence quantum distributions, fluorescence quantum yields and degrees of fluorescence polarization vs, dye concentration were determined for indocyanine green in methanol, water and aqueous albumin solution. The monomer fluorescence quantum yield is limited to a few per cent by internal conversion. In water, dimerization starts at low concentrations (approximately 3 x 10(-8) mol dm(-3)) and lowers the fluorescence quantum yield. The strong affinity of the dye to albumin shifts the onset of dimerization to higher concentrations. in methanol, dimerization is weak and closely spaced pair formation dominates at high concentrations. Dye adsorption to albumin and dye aggregation in the solvents were analysed experimentally and theoretically.
引用
收藏
页码:137 / 148
页数:12
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