Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion

被引：393

作者：

Ben-Shahar, Tom Rolef ^{[1
]}

Heeger, Sebastian ^{[1
]}

Lehane, Chris ^{[1
]}

East, Philip ^{[2
]}

Flynn, Helen ^{[3
]}

Skehel, Mark ^{[3
]}

Uhlmann, Frank ^{[1
]}

机构：

[1] Canc Res UK London Res Inst, Chromosome Segregat Lab, London WC2A 3PX, England

[2] Canc Res UK London Res Inst, Bioinformat & Biostat Serv, London WC2A 3PX, England

[3] Canc Res UK London Res Inst, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

来源：

SCIENCE | 2008年 / 321卷 / 5888期

关键词：

D O I：

10.1126/science.1157774

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork- associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation- mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1- dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1- independent.

引用

页码：563 / 566

页数：4

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