Systemic and mucosal immune responses in mice after rectal and vaginal immunization with HIV-DNA vaccine

被引:30
作者
Hamajima, K [1 ]
Hoshino, Y [1 ]
Xin, KQ [1 ]
Hayashi, F [1 ]
Tadokoro, K [1 ]
Okuda, K [1 ]
机构
[1] Yokohama City Univ, Sch Med, Dept Bacteriol, Yokohama, Kanagawa 2360004, Japan
关键词
D O I
10.1006/clim.2001.5141
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We examined the feasibility of inducing local and systemic human immunodeficiency virus (HIV)-specific immune responses by rectal and vaginal application of an HIV-DNA vaccine. Mice were immunized with an HIV-DNA vaccine preparation via a rectal or vaginal route. After several applications, HIV-specific antibodies were detected in sera, fecal extract solutions, and vaginal washes, and these antibodies were potent in inhibiting the syncytium formation of a CD4-positive human T cell line by a cell line capable of inducing HIV-1 infection. Spleen cells from rectally and vaginally immunized mice showed antigen-mediated IFN-gamma-inducing activity. In addition, with rectal immunization, mononuclear cells from both the spleen and the regional lymph nodes of the rectal region were found to be potent at inducing a cytotoxic T lymphocyte response. These humoral and cell-mediated immune responses were enhanced by augmenting the vaccine with granulocyte-macrophage colony-stimulating factor-expressing plasmids or IL-12-expressing plasmid. Our results demonstrated that both rectal and vaginal immunization could induce systemic and mucosal immunity and that these responses were enhanced by the addition of the above cytokine-expressing plasmids. (C) 2001 Elsevier Science.
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收藏
页码:12 / 18
页数:7
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