Comparison of glycine and GABA actions on the zebrafish homomeric glycine receptor

被引:45
作者
Fucile, S [1 ]
de Saint Jan, D [1 ]
David-Watine, B [1 ]
Korn, H [1 ]
Bregestovski, P [1 ]
机构
[1] Inst Pasteur, INSERM U261, F-75724 Paris 15, France
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1999年 / 517卷 / 02期
关键词
D O I
10.1111/j.1469-7793.1999.0369t.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Glycine and GABA can be co-released from the same presynaptic terminals and in lower vertebrates they can activate the same glycine receptors (GlyRs). Thus we examined the effects of these two inhibitory transmitters on the homomeric GlyRs formed by the alpha Z1 subunit, of the zebrafish using two expression systems: Xenopus oocytes and the human BOSC 23 cell line. 2. The apparent affinity (EC50) of alpha Z1 for these neurotransmitters was highly variable. In Xenopus oocytes the EC50 ranged from 37 to 360 mu M (mean +/- S.D. EC50 116 +/- 75 mu M, n = 83) for glycine and from 8 to 120 mM (mean EC50 40 +/- 30 mM, n = 37) for GABA. 3. In BOSC cells the EC50 varied from 9 to 92 mu M (mean EC50 33 +/- 17 mu M, n = 19) and from 0.7 to 19.1 mM (mean EC50 4.9 +/- 4.7 mM, n = 29) for glycine and GABA, respectively. 4. GABA activated alpha Z1 GlyRs either as a weak or full agonist: its efficacy (defined as I-max,I-GABA/I-max,I-Gly) was related to EC50 by an exponential relationship. A linear relationship was observed between EC50 values for GABA and glycine. 5. In outside-out patches, GABA and glycine activated alpha Z1 with identical single-channel conductances (85-100 pS), but with different kinetics and marked effect of concentration on burst duration for glycine only. 6. In outside-out patches deactivation time constants were concentration dependent for glycine, but not for GABA. 7. Our data demonstrate that the kinetics of glycine and G-ABA interactions with alpha Z1 are different and that they determine the properties of these neurotransmitter actions on the GlyR.
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页码:369 / 383
页数:15
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