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Lysophosphatidylcholine potentiates the mitogenic activity of modified LDL for human monocyte-derived macrophages

被引：73
作者：
Sakai, M
Miyazaki, A
Hakamata, H
Sato, Y
Matsumura, T
Kobori, S
Shichiri, M
Horiuchi, S
机构：
[1] KUMAMOTO UNIV, SCH MED, DEPT BIOCHEM, KUMAMOTO 860, JAPAN
[2] KUMAMOTO UNIV, SCH MED, DEPT METAB MED, KUMAMOTO 860, JAPAN
来源：
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | 1996年 / 16卷 / 04期
关键词：
human monocyte-derived macrophage; foam cell; oxidized LDL; lysophosphatidylcholine; scavenger receptor;
D O I：
10.1161/01.ATV.16.4.600
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The growth of murine peritoneal macrophages is induced by oxidized LDL (Ox-LDL), and lysophosphatidylcholine (lysoPC) plays an important role in its mitogenic activity. In the present study, Ox-LDL-induced macrophage growth was examined with human monocyte-derived macrophages. The cell growth of human macrophages was significantly induced by Ox-LDL but not by acetylated LDL (acetyl-LDL). The treatment of acetyl-LDL with phospholipase A(2). however, led to a marked increase in its mitogenic activity, with a concomitant conversion of 75% of its phospholipids to lysoPC. The growth-stimulating activity became positive only when both acetyl-LDL and lysoPC were coincubated, although neither of them exhibited cell growth-promoting activity. These results suggest that Ox-LDL could stimulate the growth of human monocyte-derived macrophages, and lysoPC may play an essential role in the mitogenic activity of Ox-LDL.
引用
收藏
页码：600 / 605
页数：6
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