Novel multiplex PCR assay for detection of the staphylococcal virulence marker panton-valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci

被引:277
作者
McClure, JA
Conly, JM
Lau, V
Elsayed, S
Louie, T
Hutchins, W
Zhang, KY
机构
[1] Univ Calgary, Dept Pathol & Lab Med, Calgary, AB T2L 2K8, Canada
[2] Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2L 2K8, Canada
[3] Univ Calgary, Dept Med, Calgary, AB T2L 2K8, Canada
[4] Univ Calgary, Ctr Antimicrobial Resistance, Calgary Hlth Reg, Calgary Lab Serv, Calgary, AB T2L 2K8, Canada
[5] Calgary Lab Serv, Calgary, AB, Canada
关键词
D O I
10.1128/JCM.44.3.1141-1144.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.
引用
收藏
页码:1141 / 1144
页数:4
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