Novel Genipin-Cross-Linked Chitosan/Silk Fibroin Sponges for Cartilage Engineering Strategies

被引：219

作者：

Silva, Simone S. ^{[1
,2
]}

Motta, Antonella ^{[3
,4
]}

Rodrigues, Marcia T. ^{[1
,2
]}

Pinheiro, Ana F. M. ^{[1
,2
]}

Gomes, Manuela E. ^{[1
,2
]}

Mano, Joao F. ^{[1
,2
]}

Reis, Rui L. ^{[1
,2
]}

Migliaresi, Claudio ^{[3
,4
]}

机构：

[1] Univ Minho, Dept Polymer Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, Headquarters European Inst Excellence Tissue Engn, P-4806909 Caldas Das Taipas, Guimaraes, Portugal

[2] Inst Biotechnol & Bioengn, PT Govt Associated Lab, Braga, Portugal

[3] Univ Trent, Dept Mat Engn & Ind Technol, I-38050 Trento, Italy

[4] Univ Trent, INSTM Res Unit, I-38050 Trento, Italy

来源：

BIOMACROMOLECULES | 2008年 / 9卷 / 10期

关键词：

D O I：

10.1021/bm800874q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel hydrogels composed of blends of chitosan and Bombyx mori silk fibroin were cross-linked with genipin (G) and were freeze dried to obtain chitosan/silk (CSG) sponges. CSG sponges possess stable and ordered structures because of protein conformational changes from (alpha-helix/random-coil to beta-sheet structure, distinct surface morphologies, and pH/ swelling dependence at pH 3, 7.4, and 9. We investigated the cytotoxicity of CSG sponge extracts by using L929 fibroblast-like cells. Furthermore, we cultured ATDC5 cells onto the sponges to evaluate the CSG sponges' potential in cartilage repair strategies. These novel sponges promoted adhesion, proliferation, and matrix production of chondrocyte-like cells. Sponges' intrinsic properties and biological results suggest that CSG sponges may be potential candidates for cartilage tissue engineering (TE) strategies.

引用

页码：2764 / 2774

页数：11

共 53 条

[1] Collagens -: major component of the physiological cartilage matrix, major target of cartilage degeneration, major tool in cartilage repair [J].