Cadmium metallothionein gene of Tetrahymena pyriformis

被引：40

作者：

Piccinni, E

Bertaggia, D

Santovito, G

Miceli, C

Kraev, A

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

[2] Univ Camerino, Dept Mol Cellular Anim Biol, I-62032 Camerino, Italy

[3] Swiss Fed Inst Technol, Lab Biochem 3, Zurich, Switzerland

来源：

GENE | 1999年 / 234卷 / 01期

关键词：

ciliates; gene duplication; genetic code; inducible promoters; vectorette;

D O I：

10.1016/S0378-1119(99)00181-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A genomic sequence from Tetrahymena pyriformis, encoding a cadmium-induced metallothionein has been cloned. The gene encodes a transcript of 487 bases, with an intronless coding region of 324 nt, using TGA as the stop codon, TAA coding for glutamine, and the translational initiation sequence AAAATGG. Two regions of internal similarity in the coding sequence support the hypothesis that the Tetrahymena protein arose by gene duplication. The sequences of untranslated regions show some similarities with nematode MT-I and MT-2 transcripts. Sequence of 525 bases upstream of the transcription start contains a TATA box, a CAAT box reverse complement, and many short stretches partially matching the AP-1 and ACE-I binding sites, but no characteristic sequences found in other metallothionein promoters. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：51 / 59

页数：9

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