A maize-specifically expressed gene cluster in Ustilago maydis

被引:53
作者
Basse, CW [1 ]
Kolb, S [1 ]
Kahmann, R [1 ]
机构
[1] Univ Munich, Inst Genet & Mikrobiol, D-80638 Munich, Germany
关键词
D O I
10.1046/j.1365-2958.2002.02742.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The corn pathogen Ustilago maydis requires its host plant maize for development and completion of its sexual cycle. We have identified the fungal mig2-1 gene as being specifically expressed during this biotrophic stage. Intriguingly, mig2-1 is part of a gene cluster comprising live highly homologous and similarly regulated genes designated mig2-1 to mig2-5. Deletion analysis of the mig2-1 promoter provides evidence for negative and positive regulation. The predicted polypeptides; of all five genes lack significant homologies to known genes but have characteristic N-terminal secretion sequences. The secretion signals of mig2-1 and mig2-5 were shown to be functional, and secretion of a full length Mig2-1-eGFP fusion protein to the extracellular space was demonstrated. The central domains of the Mig2 proteins are highly variable whereas the C-termini are strongly conserved and share a characteristic pattern of eight cysteine residues. The mig2 gene cluster was conserved in a wide collection of U. maydis strains. Interestingly, some U. maydis isolates from South America had lost the mig2-4 gene as a result of a homologous recombination event. Furthermore, the related Ustilago scitaminea strain, which is pathogenic on sugar cane, appears to lack the mig2 cluster. We describe a model of how the mig2 cluster might have evolved and discuss its possible role in governing host interaction.
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页码:75 / 93
页数:19
相关论文
共 52 条
[1]  
AICHINGER C, 2000, THESIS LMU MUNCHEN M
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]  
Banuett F, 1996, DEVELOPMENT, V122, P2965
[4]   DIFFERENT A-ALLELES OF USTILAGO-MAYDIS ARE NECESSARY FOR MAINTENANCE OF FILAMENTOUS GROWTH BUT NOT FOR MEIOSIS [J].
BANUETT, F ;
HERSKOWITZ, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5878-5882
[5]   Characterization of a Ustilago maydis gene specifically induced during the biotrophic phase:: Evidence for negative as well as positive regulation [J].
Basse, CW ;
Stumpferl, S ;
Kahmann, R .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (01) :329-339
[6]  
BOHLMANN R, 1996, THESIS LMU MUNCHEN M
[7]  
BOLKER M, 1995, CAN J BOT, V73, pS320
[8]   THE A MATING TYPE LOCUS OF U-MAYDIS SPECIFIES CELL SIGNALING COMPONENTS [J].
BOLKER, M ;
URBAN, M ;
KAHMANN, R .
CELL, 1992, 68 (03) :441-450
[9]  
CHEN WS, 1995, ONCOL REP, V2, P5
[10]   Starvation-induced genes of the tomato pathogen Cladosporium fulvum are also induced during growth in planta [J].
Coleman, M ;
Henricot, B ;
Arnau, J ;
Oliver, RP .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (09) :1106-1109