Detection and determination of the major metabolites of [H-3]cytosine arabinoside by high-performance liquid chromatography

被引：16

作者：

Braess, J ^{[1
]}

Pfortner, J ^{[1
]}

Kaufmann, CC ^{[1
]}

Ramsauer, B ^{[1
]}

Unterhalt, M ^{[1
]}

Hiddemann, W ^{[1
]}

Schleyer, E ^{[1
]}

机构：

[1] UNIV GOTTINGEN,DEPT HEMATOL & ONCOL,D-37075 GOTTINGEN,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 676卷 / 01期

关键词：

solid-phase scintillation detection; cytosine arabinoside;

D O I：

10.1016/0378-4347(95)00372-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ion-pair high-performance liquid chromatographic assay involving solid-phase scintillation detection was established for the rapid identification and determination of all major metabolites of tritium-labelled cytosine arabinoside (Ara-C) in an in vitro system. In a single run of 50 min, Ara-C, Ara-CMP, Ara-CDP-choline, Ara-CDP, Ara-U, Ara-UMP, Ara-CTP, Ara-UDP and Ara-UTP can be measured. The method is fast, sensitive, with limits of detection ranging from 40 to 200 pg (absolute), and highly reproducible.

引用

页码：131 / 140

页数：10

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