Bone marrow stromal cells produce nerve growth factor and glial cell line-derived neurotrophic factors

被引：118

作者：

García, R

Aguiar, J

Alberti, E

de la Cuétara, K

Pavón, N

机构：

[1] Int Ctr Neurol Restorat, Havana, Cuba

[2] Ctr Genet Engn & Biotechnol, Havana, Cuba

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 316卷 / 03期

关键词：

GDNF; NGF; rat bone marrow stromal cells; RT-PCR;

D O I：

10.1016/j.bbrc.2004.02.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bone marrow stromal cells (BMSC) have attracted interest through their possible use for cell therapy in neurological diseases. Recent reports demonstrated that these cells are able to migrate and have potential for neuronal differentiation after transplantation into brain parenchyma. The objective of this work was determine whether rat BMSC express NGF and GDNF, in order to study its potential application for treatment of neurodegenerative diseases. BMSC were harvested from mate rats and cultured in DMEM supplemented with 20% fetal bovine serum. At passage 6 the total RNA was isolated using TriZol reactive. RT-PCRs to evaluate the expression of NGF and GDNF using specific primers were carried out. Our results indicate that rat BMSC have potential to produce NGF and GDNF. We have not found any report in favor of GDNF or NGF production from rat BMSC. (C) 2004 Published by Elsevier Inc.

引用

页码：753 / 754

页数：2

共 6 条

[1] Nerve growth factor is involved in the supportive effect by bone marrow-derived stromal cells of the factor-dependent human cell line UT-7 [J].