Differential Pax6 promoter activity and transcript expression during forebrain development

被引：27

作者：

Anderson, TR

Hedlund, E

Carpenter, EM

机构：

[1] Univ Calif Los Angeles, Sch Med, Mental Retardat Res Ctr, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Sch Med, Neurosci IDP, Los Angeles, CA 90024 USA

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 114卷 / 1-2期

关键词：

transcription factor; promoter usage; mouse; gene regulation; forebrain development; telencephalon; diencephalon; alternative splicing; gene expression;

D O I：

10.1016/S0925-4773(02)00051-5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three different Pax6 promoters - P0, P1, and Palpha - show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse transcription-polymerase chain reaction to detect transcripts initiated from each promoter. Promoter-specific transcripts are expressed within subdomains of total Pax6 expression, but differ from one another in their spatial localization and expression over time. Additionally, we identified a novel P0-initiated transcript and detected a developmentally regulated antisense transcript. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：171 / 175

页数：5

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