Cloning and characterization of the gene encoding Aspergillus nidulans DNA topoisomerase II

被引：11

作者：

Kim, KH ^{[1
]}

Akashi, T ^{[1
]}

Mizuguchi, I ^{[1
]}

Kikuchi, A ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Dis Mechanism & Control Res Inst, Med Mycol Lab,Showa Ku, Nagoya, Aichi 4668550, Japan

来源：

GENE | 1999年 / 236卷 / 02期

关键词：

Aspergillus nidulans; molecular cloning; topoisomerase II;

D O I：

10.1016/S0378-1119(99)00268-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have determined the complete nucleotide sequence of a 5544 bp genomic DNA fragment from Aspergillus nidulans that encodes DNA topoisomerase II (topo II). It contains a single open reading frame of 4740 bp that codes for 1579 amino acid residues with a molecular weight of 178 kDa; when expressed in Escherichia coli and Saccharomyces cerevisiae the molecular weight was 180 kDa. The gene (TOP2) is divided into three exons. Two introns, 54 bp and 60 bp in length, are located at nucleotide positions 187 and 3214 respectively. Comparison of the deduced amino acid sequence with other eukaryotic topo II sequences showed a higher degree of identity with other fungal enzymes than the human topo II alpha. One of monoclonal antibodies raised against human topo II, 6H8, can cross-react with Aspergillus topo II. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：293 / 301

页数：9

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