The Wilms tumor protein is persistently associated with the nuclear matrix throughout the cell cycle

被引：5

作者：

Dobashi, Y

Kudoh, T

Ishidate, T

Shoji, M

Toyoshima, K

Akiyama, T

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT ONCOGENE RES,SUITA,OSAKA 565,JAPAN

[2] KITASATO UNIV,SCH MED,DEPT PATHOL,SAGAMIHARA,KANAGAWA 228,JAPAN

[3] CTR ADULT DIS,OSAKA 537,JAPAN

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1997年 / 171卷 / 1-2期

关键词：

WT1; protein; nuclear matrix; cell cycle; salt extraction; DNase digestion;

D O I：

10.1023/A:1006884527897

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In order to investigate the subnuclear interactions of the WTI gene product, nuclear fractionation analyses were performed with human osteosarcoma HOS and myelogenous leukemia K562 cells, The WTI protein was tightly associated with the nucleus and was resistant to high-salt or derergent extraction and DNase I digestion. Both the expression level and stability of WTI and its resistance to high salt and DNase I treatments remained constant during the cell cycle. In addition, human WTI ectopically expressed in mouse NIH3T3 cells was also resistant to these treatments. These results suggest that WTI functions in tight association with the nuclear matrix.

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页码：121 / 126

页数：6

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