Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses

被引：82

作者：

Hangalapura, BN ^{[1
]}

Kaiser, MG

van der Poel, JJ

Parmentier, HK

Lamont, SJ

机构：

[1] Wageningen Univ, Wageningen Inst Anim Sci, Adaptat Physiol Grp, NL-6709 PG Wageningen, Netherlands

[2] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA

[3] Wageningen Univ, Inst Anim Sci, Breeding & Genet Grp, Wageningen, Netherlands

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2006年 / 30卷 / 05期

关键词：

cold stress; complete Freund's adjuvant; chicken lines; cytokine; mRNA; real-time quantitative reverse transcriptase-polymerase chain reaction; peripheral blood leukocytes;

D O I：

10.1016/j.dci.2005.07.001

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The effects of cold stress, immunization and genetic selection on the expression of mRNA for cytokine genes in poultry have not been completely elucidated. Therefore, in the present experiment, using real-time quantitative RT-PCR, we evaluated the effect of cold stress and immunization with complete Freund's adjuvant (CFA) on expression of mRNA for pro-inflammatory (interleukin-1 beta [IL-I beta], IL-6, IL-12 beta), Th-1 (IFN-gamma and IL-2), and Th-2 (IL-4 and IL-10) cytokine genes in peripheral blood leukocytes (PBL) of chicken lines divergently selected for either high or low antibody responses. Irrespective of the duration, cold stress enhanced expression of mRNA for IL-1 beta, IL-6, IL-12 beta and IL-4 cytokine genes in both selection lines. These results indicate that cold stress stimulates both the innate and parts of the adaptive cellular immune system. Immunization with CFA resulted in higher expression of mRNA for pro-inflammatory cytokines and lower expression of mRNA for both Th-1 and Th-2 cytokines. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：503 / 511

页数：9

共 37 条

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[Anonymous], JMP STAT GRAPH GUID

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