Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro

被引:25
作者
Bolton, AJ
Martin, GD
Osborne, MP
Wallis, TS
Stephen, J
机构
[1] Univ Birmingham, Sch Biol Sci, Microbial Mol Genet & Cell Biol Grp, Birmingham B15 2TT, W Midlands, England
[2] Univ Birmingham, Sch Med, Dept Physiol, Birmingham B15 2TT, W Midlands, England
[3] Inst Anim Hlth, Newbury, Berks, England
关键词
D O I
10.1099/00222615-48-9-801
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness that was comparable to that of strain TML, One isolate (GM3) was apparently substantially less invasive; electron microscopy showed this strain to be histotoxic - the probable reason for its reduced recovery from ileal mucosa and thus apparent 'low' invasiveness, Salmonella serotype Choleraesuis strain A50, isolated from a case of systemic salmonellosis in pigs, and serotype Dublin strain 3246, isolated from a case of systemic salmonellosis in calves, were also examined. Dublin strain 3246, when grown at 37 degrees C and used immediately in the invasion assay, damaged the mucosa in a manner similar to that of Typhimurium strain GM3, whereas Dublin strain 3246 grown at 37 degrees C and stored overnight at 4 degrees C did not. This was reflected in an apparently lower invasiveness of freshly grown organisms compared with that of organisms stored at 4 degrees C. In contrast, the histotoxicity of Typhimurium strain GM3 was not affected by storage at 4 degrees C. When stored at 4 degrees C, the levels of invasiveness of Choleraesuis strain A50 and Dublin strain 3246 were not significantly different from each other or from Typhimurium strain TML.
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页码:801 / 810
页数:10
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