Isolation and identification of a major urinary canthaxanthin metabolite in rats

被引：14

作者：

Bausch, J ^{[1
]}

Liechti, H ^{[1
]}

Oesterhelt, G ^{[1
]}

Kistler, A ^{[1
]}

机构：

[1] F Hoffmann La Roche Ltd, CH-4070 Basel, Switzerland

来源：

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH | 1999年 / 69卷 / 04期

关键词：

carotenoids; canthaxanthin; metabolism; urinary metabolite; rat;

D O I：

10.1024/0300-9831.69.4.268

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The urinary metabolic pattern after administration of the radiolabeled non-provitamin A carotenoid canthaxanthin was investigated in rats. In the rather complex HPLC urinary metabolic pattern a fraction was found which was conjugated. Deconjugation of the polar conjugates with glusulase, purification of the metabolite with HPLC and identification with GC-MS and NMR revealed that it was 3-hydroxy-4-oxo-7,8-dihydro-beta-ionone. This structure was confirmed by comparisons with HPLC retention times, UV/VIS- and NMR-spectroscopy and GC-MS of the synthesized compound.

引用

页码：268 / 272

页数：5

共 7 条

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