Physcomitrella patens gene/cDNA fragments related to genes encoding protein Ser/Thr phosphatases

被引：8

作者：

Andreeva, AV ^{[1
]}

Kutuzov, MA

机构：

[1] Oxford Brookes Univ, Res Sch Biol & Mol Sci, Oxford OX3 0BP, England

[2] CEA Grenoble, DBMS, Lab Biophys Mol & Cellulaire, F-38054 Grenoble 9, France

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1999年 / 155卷 / 02期

关键词：

Physcomitrella patens; protein phosphorylation; protein Ser/Thr phosphatase; polymerase chain reaction;

D O I：

10.1016/S0176-1617(99)80001-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A number of higher plant protein Ser/Thr phosphatase (PP) genes have been recently identified encoding PP1, PP2A, PPX and PP7. Using polymerase chain reaction on Physcomitrella patens (Hedw.) B.S.G. (Funariales) genomic and cDNA, we have analysed the protein Ser/Thr phosphatase composition in this lower plant. The analysis revealed the presence of PP1 (4 isoforms), PP2A (4 isoforms), PPX (1 isoform), PP5 (I isoform) and putative PP7-related phosphatases (3 isoforms). In addition, we identified a cDNA fragment homologous to <<expressed sequence tags>> from Arabidopsis thaliana and Oryza sativa, which probably encode as yet unidentified phosphatases distantly related to the PPP family. These results provide first structural data about the protein Ser/Thr phosphatases in lower land plants.

引用

页码：153 / 158

页数：6

共 43 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

Andreeva AV, 1997, BIOORG KHIM+, V23, P486

[3]

Andreeva AV, 1999, BIOCHEMISTRY-MOSCOW+, V64, P228

[4]

Andreeva AV, 1998, BIOCHEM MOL BIOL INT, V44, P703

[5]

ANDREEVA AV, 1999, IN PRESS PHYSL PLANT

[6]

BECKER W, 1994, J BIOL CHEM, V269, P22586

[7] ABSCISIC-ACID AND DESICCATION TOLERANCE IN MOSSES [J].

BOPP, M ;

WERNER, O .

BOTANICA ACTA, 1993, 106 (02) :103-106

[8] PPX, A NOVEL PROTEIN SERINE THREONINE PHOSPHATASE LOCALIZED TO CENTROSOMES [J].

BREWIS, ND ;

STREET, AJ ;

PRESCOTT, AR ;

COHEN, PTW .

EMBO JOURNAL, 1993, 12 (03) :987-996

[9] MOLECULAR CHARACTERIZATION OF A 4TH ISOFORM OF THE CATALYTIC SUBUNIT OF PROTEIN PHOSPHATASE-2A FROM ARABIDOPSIS-THALIANA [J].

CASAMAYOR, A ;

PEREZCALLEJON, E ;

PUJOL, G ;

ARINO, J ;

FERRER, A .

PLANT MOLECULAR BIOLOGY, 1994, 26 (01) :523-528

[10] POLYMERASE CHAIN-REACTIONS USING SACCHAROMYCES, DROSOPHILA AND HUMAN DNA PREDICT A LARGE FAMILY OF PROTEIN SERINE THREONINE PHOSPHATASES [J].

CHEN, MX ;

CHEN, YH ;

COHEN, PTW .

FEBS LETTERS, 1992, 306 (01) :54-58

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