Endophytic expression of nif genes of Azoarcus sp strain BH72 in rice roots

被引:115
作者
Egener, T [1 ]
Hurek, T [1 ]
Reinhold-Hurek, B [1 ]
机构
[1] Max Planck Inst Terr Mikrobiol, Arbeitsgrp Symbioseforsch, D-35043 Marburg, Germany
关键词
ammonium; oxygen tolerance; repression;
D O I
10.1094/MPMI.1999.12.9.813
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptional fusions of gusA and gfp to the nifH gene as well as immunogold labeling with antibodies against the iron protein of nitrogenase revealed high nitrogenase gene expression levels of the endophyte Azoarcus sp. strain BH72 inside infected rice roots (Oryza sativa) in the laboratory, Thus, environmental conditions inside rice roots are permissive for endophytic nitrogen fixation in bacterial microcolonies in the aerenchyma.
引用
收藏
页码:813 / 819
页数:7
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