Heterogeneity of Photosystem I reaction centers in barley leaves as related to the donation from stromal reductants

The light-response curves of P700 oxidation and time-resolved kinetics of P700(+) dark re-reduction were studied in barley leaves using absorbance changes at 820 nm. Leaves were exposed to 45 degreesC and treated with either diuron or diuron plus methyl viologen (MV) to prevent linear electron flow from PS II to PS I and ferredoxin-dependent cyclic electron flow around PS I. Under those conditions, P700(+) could accept electrons solely from soluble stromal reductants. P700 was oxidized under weak far-red light in leaves treated with diuron plus MV, while identical illumination was nearly ineffective in diuron-treated leaves in the absence of MV. When heat-exposed leaves were briefly illuminated with strong far-red light, which completely oxidized P700, the kinetics of P700(+) dark reduction was fitted by a single exponential term with half-time of about 40 ms. However, two first-order kinetic components of electron flow to P700(+) (fast and slow) were found after prolonged leaf irradiation. The light-induced modulation of the kinetics of P700(+) dark reduction was reversed following dark adaptation. The fast component (half time of 80-90 ms) was 1.5 larger than the slow one (half time of about 1 s). No kinetic competition occurred between two pathways of electron donation to P700(+) from stromal reductants. This suggests the presence of two different populations of PS I.