16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (necropolis of Carmona, Seville, Spain)

被引：75

作者：

Heyrman, J

Swings, J

机构：

[1] Univ Ghent, Dept Biochem Physiol & Microbiol WE10V, Microbiol Lab, B-9000 Ghent, Belgium

[2] BCCM LMG Culture Collect, Ghent, Belgium

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2001年 / 24卷 / 03期

关键词：

165 rDNA sequencing; fatty acid analysis; biodeterioration; mural paintings;

D O I：

10.1078/0723-2020-00048

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacteria were isolated from damaged. mural paintings of the Servilia tomb (necropolis of Carmona, Seville, Spain). Selected strains, representative for different clusters of isolates with similar fatty acid profiles, were analysed by 165 rDNA sequence analysis. Bacillus is the dominant genus among the isolates: members of the rRNA species complexes of B. megaterium, B. pumilus and B. firmus were found as well as several other Bacillus species. One group of halotolerant isolates falls in the Bacillus sensu lato group, with closest relatedness to the genera Salibacillus anal Virgibacillus. Other genera found are Arthrobacter, Micrococcus, Streptomyces, Sphingomonas, Paenibacillus, and a genus closely related to Paracraurococcus. Many isolates showed low 165 rDNA sequence similarities with the closest related database entries, a strong indication for the presence of several new species among the isolates.

引用

页码：417 / 422

页数：6

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