Human photoreceptor transplantation in retinitis pigmentosa - A safety study

被引：90

作者：

Kaplan, HJ ^{[1
]}

Tezel, TH ^{[1
]}

Berger, AS ^{[1
]}

Wolf, ML ^{[1
]}

DelPriore, LV ^{[1
]}

机构：

[1] WASHINGTON UNIV, SCH MED, DEPT BIOCHEM & MOL BIOPHYS, ST LOUIS, MO 63110 USA

来源：

ARCHIVES OF OPHTHALMOLOGY | 1997年 / 115卷 / 09期

关键词：

D O I：

10.1001/archopht.1997.01100160338012

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Objective: To establish the technical feasibility and safety of photoreceptor Methods: A sheet of human photoreceptor cells was harvested from 2 human cadaveric eyes with a vibratome and transplanted into the subretinal spaces of 2 patients with advanced retinitis pigmentosa and visual acuity of no light perception by means of submacular surgery techniques. Preoperative and postoperative electrophysiologic testing, fundus photography, fluorescein angiography, and scanning laser ophthalmoscopy were performed. Results: Twelve months after photoreceptor transplantation, the visual acuity of each patient remained no light perception. The temporal edge of the retinotomy in I patient was folded but was not associated with a retinal detachment. The patients were not immunosuppressed, and there was no evidence of rejection of the allogeneic transplant. Cystoid macular edema, uveitis, and. macular pucker were not observed. Conclusion: A sheet of adult human photoreceptor cells can be harvested from human cadaveric eyes and safely transplanted to the subretinal spaces of patients with retinitis pigmentosa without systemic immunosuppression.

引用

页码：1168 / 1172

页数：5

共 24 条

[1] FUNCTION AND STRUCTURE IN RETINAL TRANSPLANTS [J].

ADOLPH, AR ;

ZUCKER, CL ;

EHINGER, B ;

BERGSTROM, A .

JOURNAL OF NEURAL TRANSPLANTATION & PLASTICITY, 1994, 5 (03) :147-161

[2] MUTATION ANALYSIS OF THE ROM1 GENE IN RETINITIS-PIGMENTOSA [J].

BASCOM, RA ;

LIU, L ;

HECKENLIVELY, JR ;

STONE, EM ;

MCINNES, RR .

HUMAN MOLECULAR GENETICS, 1995, 4 (10) :1895-1902

[3] Photoreceptor cell rescue in retinal degeneration (rd) mice by in vivo gene therapy [J].

Bennett, J ;

Tanabe, T ;

Sun, DX ;

Zeng, Y ;

Kjeldbye, H ;

Gouras, P ;

Maguire, AM .

NATURE MEDICINE, 1996, 2 (06) :649-654

[4] Retinitis pigmentosa: Unfolding its mystery [J].

Berson, EL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (10) :4526-4528

[5] INTRARETINAL GRAFTING RESTORES VISUAL FUNCTION IN LIGHT-BLINDED RATS [J].

DELCERRO, M ;

ISON, JR ;

BOWEN, GP ;

LAZAR, E ;

DELCERRO, C .

NEUROREPORT, 1991, 2 (09) :529-532

[6] A POINT MUTATION OF THE RHODOPSIN GENE IN ONE FORM OF RETINITIS-PIGMENTOSA [J].

DRYJA, TP ;

MCGEE, TL ;

REICHEL, E ;

HAHN, LB ;

COWLEY, GS ;

YANDELL, DW ;

SANDBERG, MA ;

BERSON, EL .

NATURE, 1990, 343 (6256) :364-366

[7] MUTATIONS IN THE GENE ENCODING THE ALPHA-SUBUNIT OF THE ROD CGMP-GATED CHANNEL IN AUTOSOMAL RECESSIVE RETINITIS-PIGMENTOSA [J].

DRYJA, TP ;

FINN, JT ;

PENG, YW ;

MCGEE, TL ;

BERSON, EL ;

YAU, KW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) :10177-10181

[8] A 3-BASE-PAIR DELETION IN THE PERIPHERIN-RDS GENE IN ONE FORM OF RETINITIS-PIGMENTOSA [J].

FARRAR, GJ ;

KENNA, P ;

JORDAN, SA ;

KUMARSINGH, R ;

HUMPHRIES, MM ;

SHARP, EM ;

SHEILS, DM ;

HUMPHRIES, P .

NATURE, 1991, 354 (6353) :478-480

[9]

GOURAS P, 1991, Journal of Neural Transplantation and Plasticity, V2, P91

[10]

HAUGLAND RP, 1994, HDB FLUORESCENT PROB, P172

← 1 2 3 →