Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers

被引：6

作者：

Vroom, Jonathan A. ^{[1
]}

Wang, Gifford L. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

来源：

BIOTECHNIQUES | 2008年 / 44卷 / 07期

关键词：

D O I：

10.2144/000112808

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We, have developed a modular method of plasmid construction that can join multiple DNA components in a single reaction. A nicking enzyme is used to create 5' and 3' overhangs on PCR-generated DNA components. Without the use of ligase or restriction enzymes, components are joined using oligonucleotide linkers that recognize the overhangs. By specifying the sequences of the linkers, desired components can be assembled in any combination and order to generate different plasmid vectors.

引用

页码：924 / 926

页数：3

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