Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress

被引：139

作者：

Li, TK ^{[1
]}

Chen, AY ^{[1
]}

Yu, C ^{[1
]}

Mao, Y ^{[1
]}

Wang, HM ^{[1
]}

Liu, LF ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

来源：

GENES & DEVELOPMENT | 1999年 / 13卷 / 12期

关键词：

TOP2; H2O2; VM-26; oxidative stress; apoptosis;

D O I：

10.1101/gad.13.12.1553

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is known to induce oxidative stress and apoptosis. U937 cells treated with H2O2 were shown to produce high molecular weight (HMW) DNA fragments similar to 50 to 100 kb in size in <1 min. The formation of these HMW DNA fragments is reversible and shown to be mediated by DNA topoisomerase II (TOP2). Following this initial event, formation of irreversible HMW DNA fragments and nucleosomal ladders occurs. Our results thus demonstrate a potential role of TOP2 in oxidative damage of DNA and apoptotic cell death.

引用

页码：1553 / 1560

页数：8

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