A comparison between radiometric and fluorimetric methods for measuring SSAO activity

被引：6

作者：

Gella, Alejandro ^{[1
]}

Sole, Montse ^{[2
]}

Bolea, Irene ^{[2
]}

Ventriglia, Mariacarla ^{[3
,4
]}

Siotto, Mariacristina ^{[5
]}

Durany, Nuria ^{[1
]}

Squitti, Rosanna ^{[3
,4
]}

Unzeta, Mercedes ^{[2
]}

机构：

[1] Univ Int Catalunya, Fac Med, Dept Basic Sci, Sant Cugat Del Valles, Spain

[2] Univ Autonoma Barcelona, Fac Med, Dept Bioquim & Biol Mol, Inst Neurociencies, E-08193 Barcelona, Spain

[3] Campus Biomed Univ, Dept Neurol, Rome, Italy

[4] Fatebenefratelli Hosp, AFaR, Dept Neurosci, Rome, Italy

[5] Don Carlo Gnocchi Fdn ONLUS, Milan, Italy

来源：

JOURNAL OF NEURAL TRANSMISSION | 2013年 / 120卷 / 06期

关键词：

Biomarkers; Fluorimetric; Method comparison; Radiometric; Semicarbazide-sensitive amine oxidase; SENSITIVE AMINE OXIDASE; ADHESION PROTEIN-1; ALZHEIMERS-DISEASE; DIABETES-MELLITUS; VAP-1/SSAO; ENZYMES;

D O I：

10.1007/s00702-013-0987-z

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

100204 [神经病学];

摘要：

Semicarbazide-sensitive amine oxidase (SSAO) metabolizes the oxidative deamination of primary aromatic and aliphatic amines. The final cytotoxic products of its catalysis contribute to diseases involving vascular degeneration. The increasing interest in measuring SSAO activity has led to the development of several different methods. Herein, we compare SSAO activity results obtained with radiometric and fluorimetric methods in 49 plasma samples. Although not interchangeable, a significant correlation was obtained between methods. Considering these limitations, the fluorimetric method might replace the radioisotopic one.

引用

页码：1015 / 1018

页数：4

共 20 条

[1]

Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner [J].

Abella, A ;

García-Vicente, S ;

Viguerie, N ;

Ros-Baró, A ;

Camps, M ;

Palacín, M ;

Zorzano, A ;

Marti, L .

DIABETOLOGIA, 2004, 47 (03) :429-438

[2]

STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].

BLAND, JM ;

ALTMAN, DG .

LANCET, 1986, 1 (8476) :307-310

[3]

Plasma semicarbazide-sensitive amine oxidase is elevated in patients with congestive heart failure [J].

Boomsma, F ;

vanVeldhuisen, DJ ;

deKam, PJ ;

ManintVeld, AJ ;

Mosterd, A ;

Lie, KI ;

Schalekamp, MADH .

CARDIOVASCULAR RESEARCH, 1997, 33 (02) :387-391

[4]

PLASMA SEMICARBAZIDE-SENSITIVE AMINE OXIDASE ACTIVITY IS ELEVATED IN DIABETES-MELLITUS AND CORRELATES WITH GLYCOSYLATED HEMOGLOBIN [J].

BOOMSMA, F ;

DERKX, FHM ;

VANDENMEIRACKER, AH ;

MANINTVELD, AJ ;

SCHALEKAMP, MADH .

CLINICAL SCIENCE, 1995, 88 (06) :675-679

[5]

Semicarbazide-sensitive amine oxidase (SSAO) from human and bovine cerebrovascular tissues: biochemical and immunohistological characterization [J].

Castillo, V ;

Lizcano, JM ;

Visa, J ;

Unzeta, M .

NEUROCHEMISTRY INTERNATIONAL, 1998, 33 (05) :415-423

[6]

Overexpression of semicarbazide sensitive amine oxidase in the cerebral blood vessels in patients with Alzheimer's disease and cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy [J].

Ferrer, I ;

Lizcano, JM ;

Hernández, M ;

Unzeta, M .

NEUROSCIENCE LETTERS, 2002, 321 (1-2) :21-24

[7]

Human plasma semicarbazide sensitive amine oxidase (SSAO), β-amyloid protein and aging [J].

Hernandez, MD ;

Esteban, M ;

Szabo, P ;

Boada, M ;

Unzeta, M .

NEUROSCIENCE LETTERS, 2005, 384 (1-2) :183-187

[8]

VAP-1/SSAO Plasma Activity and Brain Expression in Human Hemorrhagic Stroke [J].

Hernandez-Guillamon, Mar ;

Sole, Montse ;

Delgado, Pilar ;

Garcia-Bonilla, Lidia ;

Giralt, Dolors ;

Boada, Cristina ;

Penalba, Anna ;

Garcia, Sandra ;

Flores, Alan ;

Ribo, Marc ;

Alvarez-Sabin, Jose ;

Ortega-Aznar, Arantxa ;

Unzeta, Mercedes ;

Montaner, Joan .

CEREBROVASCULAR DISEASES, 2012, 33 (01) :55-63

[9]

Plasma VAP-1/SSAO Activity Predicts Intracranial Hemorrhages and Adverse Neurological Outcome After Tissue Plasminogen Activator Treatment in Stroke [J].

Hernandez-Guillamon, Mar ;

Garcia-Bonilla, Lidia ;

Sole, Montse ;

Sosti, Victoria ;

Pares, Mireia ;

Campos, Mireia ;

Ortega-Aznar, Arantxa ;

Dominguez, Carmen ;

Rubiera, Marta ;

Ribo, Marc ;

Quintana, Manolo ;

Molina, Carlos A. ;

Alvarez-Sabin, Jose ;

Rosell, Anna ;

Unzeta, Mercedes ;

Montaner, Joan .

STROKE, 2010, 41 (07) :1528-1535

[10]

Serum semicarbazide-sensitive amine oxidase (SSAO) activity is an independent marker of carotid atherosclerosis [J].

Karádi, I ;

Mészáros, Z ;

Csányi, A ;

Szombathy, T ;

Hosszúfalusi, N ;

Romics, L ;

Magyar, K .

CLINICA CHIMICA ACTA, 2002, 323 (1-2) :139-146

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