The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum

被引:18
作者
Bastida, CM
Tejada, F
Cremades, A
Peñafiel, R [1 ]
机构
[1] Univ Murcia, Sch Med, Dept Biochem, Murcia 30100, Spain
[2] Univ Murcia, Sch Med, Dept Biol Mol, Murcia 30100, Spain
[3] Univ Murcia, Sch Med, Dept Pharmacol, Murcia 30100, Spain
关键词
ornithine decarboxylase; polyamines; alpha-difluoromethylornithine; progesterone; corpus luteum; mouse ovary;
D O I
10.1016/S0006-291X(02)00191-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ovarian progesterone secretion during the diestrus stage of the estrous cycle is produced by luteal cells derived from granulosa and thecal cells after the differentiation process that follows ovulation. Our results show that blockade of the preovulatory rise of ovarian ornithine decarboxylase (ODC). a key enzyme in polyamine biosynthesis. by treatment with the specific inhibitor alpha-difluoromethylornithine (DFMO) leads to a significant decrease in the ovarian progesterone content and a dramatic fall in the plasma levels of this hormone during the following diestrus. The same inhibition was produced in spite of the fact that both luteinizing and follicle stimulating hormones were given concomitantly with DFMO. On the other hand. the acute rise in the plasma progesterone levels observed after administration of human chorionic gonadotropin to mice at different periods of the estrous cycle was not affected by DFMO administration. Our results indicate that although elevated levels of ODC are not required for acute ovarian steroidogenesis. the preovulatory peak of ovarian ODC activity observed in the evening of proestrus may be critical for the establishment of a constitutive steroidogenic pathway and progesterone secretion by the corpus luteum during the diestrus stage of the murine estrous Cycle. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:106 / 111
页数:6
相关论文
共 44 条
[1]   PERMISSIVE ROLE FOR ORNITHINE DECARBOXYLASE AND PUTRESCINE IN THE LUTEINIZING-HORMONE SURGE [J].
ASLAM, M ;
NICHOLSON, S ;
GILLHAM, B ;
JONES, M .
NEUROENDOCRINOLOGY, 1987, 45 (06) :473-478
[2]   Cell transformation, invasion, and angiogenesis: A regulatory role for ornithine decarboxylase and polyamines? [J].
Auvinen, M .
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1997, 89 (08) :533-537
[3]  
BASTIDA CM, 2000, BIOL CLIN PERSPECT
[4]   Thirteen-week oral toxicity study of difluoromethylornithine in combination with tamoxifen citrate in female dogs [J].
Brown, AP ;
Morrissey, RL ;
Crowell, JA ;
Levine, BS .
CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1999, 43 (06) :479-488
[5]   Difluoromethylornithine in combination with tamoxifen in female rats: 13-week oral toxicity study [J].
Brown, AP ;
Morrissey, RL ;
Crowell, JA ;
Levine, BS .
CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1999, 44 (06) :475-483
[6]   PLASMA CONCENTRATION OF LH, FSH, PROLACTIN, PROGESTERONE AND ESTRADIOL-17BETA THROUGHOUT 4-DAY ESTROUS-CYCLE OF RAT [J].
BUTCHER, RL ;
COLLINS, WE ;
FUGO, NW .
ENDOCRINOLOGY, 1974, 94 (06) :1704-1708
[7]  
Cohen S.S., 1998, GUIDE POLYAMINES
[8]  
Feith DJ, 2001, CANCER RES, V61, P6073
[9]   OVARIAN-FUNCTION IN THE RAT FOLLOWING IRREVERSIBLE INHIBITION OF L-ORNITHINE DECARBOXYLASE [J].
FOZARD, JR ;
PRAKASH, NJ ;
GROVE, J .
LIFE SCIENCES, 1980, 27 (23) :2277-2283
[10]   INHIBITION OF MURINE EMBRYONIC-DEVELOPMENT BY ALPHA-DIFLUOROMETHYLORNITHINE, AN IRREVERSIBLE INHIBITOR OF ORNITHINE DECARBOXYLASE [J].
FOZARD, JR ;
PART, ML ;
PRAKASH, NJ ;
GROVE, J .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1980, 65 (04) :379-391