Bone marrow fibroblasts induce expression of PI3K/NF-κB pathway genes and a pro-angiogenic phenotype in CLL cells

被引：58

作者：

Edelmann, J. ^{[1
]}

Klein-Hitpass, L. ^{[2
]}

Carpinteiro, A. ^{[1
,3
]}

Fuehrer, A. ^{[1
]}

Sellmann, L. ^{[1
,2
]}

Stilgenbauer, S. ^{[4
]}

Duehrsen, U. ^{[1
]}

Duerig, J. ^{[1
]}

机构：

[1] Univ Duisburg Essen, Univ Hosp, Dept Hematol, D-45122 Essen, Germany

[2] Univ Duisburg Essen, Univ Hosp, Inst Cell Biol, D-45122 Essen, Germany

[3] Univ Duisburg Essen, Univ Hosp, Inst Mol Biol, D-45122 Essen, Germany

[4] Univ Ulm, Ulm, Germany

来源：

LEUKEMIA RESEARCH | 2008年 / 32卷 / 10期

关键词：

CLL; hematopoietic microenvironment; neoangiogenesis; osteopontin;

D O I：

10.1016/j.leukres.2008.03.003

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Microarray-based gene expression profiling (GEP) was used to study how stroma modulates the survival of CLL cells in an in vitro coculture model employing the murine fibroblast cell line M2-10B4. CLL cells cultured in direct contact with the stromal layer (STR) showed a significantly better survival than cells cultured in transwell (TW) inserts above the M2-10B4 cells. STR as compared to TW conditions induced a significant up-regulation of PI3K/NF-kappa B pro-survival pathway genes and mediated a pro-angiogenetic switch in the CLL cells by up-regulation of vascular endothelial growth factor (VEGF) and osteopontin (OPN) and down-regulation of the anti-angiogenefic molecule thrombospondin-1 (TSP-1). (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1565 / 1572

页数：8

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