The effect of charge on cellular uptake and phototoxicity of polylysine chlorin(e6) conjugates

被引:85
作者
Soukos, NS [1 ]
Hamblin, MR [1 ]
Hasan, T [1 ]
机构
[1] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,WELLMAN LABS PHOTOMED,BOSTON,MA 02114
关键词
D O I
10.1111/j.1751-1097.1997.tb01916.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of charge on the cellular uptake, localization and phototoxicity of conjugates between chlorin(e6) (c(e6)) and poly-L-lysine was studied in vitro, These conjugates (average MW 35-55 kDa) were synthesized to have polycationic, polyanionic or neutral charges, Two human cell lines (A431 epidermoid carcinoma cells and EA.hy926 hybrid endothelial cells) were studied and the cellular uptake of c(e6) delivered by the conjugates of varying charge and free c(e6) was measured at conjugate c(e6) equivalent concentrations up to 0.4 mu M. Uptake was time and concentration dependent and temperature dependent in the case of neutral and anionic conjugates, Relative uptake at 6 h for A431 cells was 73:15:4:1 and for EA.hy926 cells was 63:11:3:1 for cationic, anionic, neutral and free c(e6), respectively, but EA.hy926 cells took up 1.5-2 times as much c(e6) from all the conjugates as A431 cells, Localization as studied by fluorescence microscopy indicated that the cationic conjugate was in aggregates bound to the plasma membrane, while the other forms were internalized in organelles and membranes, Phototoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay after irradiation with 5-20 J cm(-2) of 666 nm light, In contrast to the uptake, the order of phototoxicity for both cell types per mole of c(e6) uptake per cell was neutral much greater than anionic > cationic > free c(e6), Polymeric c(e6) conjugates bearing positive, negative and neutral charges may have different tissue-localizing properties and could play a role in photodynamic therapy.
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收藏
页码:723 / 729
页数:7
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