A simple optogenetic system for behavioral analysis of freely moving small animals

被引：11

作者：

Kawazoe, Yuya ^{[1
]}

Yawo, Hiromu ^{[2
]}

Kimura, Koutarou D. ^{[1
]}

机构：

[1] Osaka Univ, Dept Biol Sci, Toyonaka, Osaka 5600043, Japan

[2] Tohoku Univ, Grad Sch Life Sci, Dept Dev Biol & Neurosci, Sendai, Miyagi 9808577, Japan

来源：

NEUROSCIENCE RESEARCH | 2013年 / 75卷 / 01期

关键词：

Optogenetics; Behavior; LED; Channelrhodopsin; C; elegans; CCD camera; CAENORHABDITIS-ELEGANS;

D O I：

10.1016/j.neures.2012.04.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We present a new and simple optogenetic system for the behavioral analysis of small animals. This system includes a strong LED ring array, a high-resolution CCD camera, and the improved channelrhodopsin ChRGR. We used the system for behavioral analysis with the nematode Caenorhabditis elegans as a model, and we found that it can stimulate ChRGR expressed in the body wall muscles of the animals to modulate the behavior. Our results indicate that this system may be suitable for optogenetic behavioral analysis of freely moving small animals under various conditions to understand the principles underlying brain functions. (C) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：65 / 68

页数：4

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